Formation of Bio-interface by Patterned Negative Ion Implantation Treatment

通过图案化负离子注入处理形成生物界面

• 批准号: 14350020
• 负责人: TSUJI Hiroshi
• 金额: $ 9.47万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14350020/
• 关键词: Negative ion implantation; Contact angle measurement; Nerve cell attachment; Artificial nerve network; Bio-interface; Neurite extension property; External electric impulse; Induction of generation; 神経細胞接着性; 人為的神経回路網; 神経再生; 再生医療

(1)Formation of nerve cell network by negative ion implantation :We implanted carbon negative ions into various polymer materials of polystyrene PS, silicone rubber SR and polyimide PI, which were spin-coated on glass, through a pattern mask with 87 slits (aperture width of 50μm). Attachment properties of Nerve cells PC-12h (rat adrenal pheochromocytoma nerve cell) were investigated on the various C-implanted polymers. Under conditions of ion energy more then 10 keV and dose more than 3x10^<15> ions/cm^2, the cells showed selective attachment on ion-implanted region for polystyrene and silicone rubber. On the other hand, they did not show it on the polyimide. Under the low energy condition of 5 keV, however, nerve cells showed the selective attachment on implanted area of polyimide. Neurite from the cell body also showed selective extension over the implanted area for PS,SR, and PI as well as cell attachment properties.(2)Surface controls of wettability and protein adsorption on polyme … More rs by negative ion implantation :We applied to measure the contract angle of water on ion-implanted various polymers by bubble method in water as well as water-drop method in air. We found that the implanted surface required many hours in water and culture medium to stabilize it surface state. Therefore, the bubble method is suitable way for evaluation of cell attachment properties. Decrease of contact angle on the surface, i.e., becoming hydrophilic was obtained were by carbon negative ion implantation : about 10° for polystyrene and 20° for silicone rubber. On the other hand, the polyimide showed an increase of contact angle, i.e., hydrophobic, about 15° by implantation. Protein adsorption was also tested for nerve growth factor NGF,FGF, and gelatin after dipping in protein-PBS solution. Nitrogen amount (from the protein) on C-implanted PS and SR surface was detected much more than that on un-implanted area by XPS analysis. This means C-implanted PS and SR were able to adsorb protein and then result selective attachment of nerve cells and neurite.(3)Response of cells selectively attached on C-implanted area of PS against externally applied electric impulse from conductive coupling electrode pad below the PS film :We investigate the outgrow of neurite from attached cells by externally applied electric impulse from conductive coupling pad. We obtained preferential outgrow of neurite from cells toward positive electric pulse electrode rather than the other electrode. This means the possibility of artificially control of connecting pattern among cells with axons. By using patch clump method, excitation of nerve cells was confirmed to be induced by the externally applied electric impulse with inductive coupling. As a result, the bio-interface between bio-nerve system and external semiconductor circuit was able to be formed by using our developed negative ion implantation method. Less

（1）通过负离子注入形成神经细胞网络：我们通过具有87个狭缝（孔径宽度为50 μ m）的图案掩模将碳负离子注入到旋涂在玻璃上的聚苯乙烯PS、硅橡胶SR和聚酰亚胺PI的各种聚合物材料中。本文研究了神经细胞PC-12h（大鼠肾上腺嗜铬细胞瘤神经细胞）在各种C-植入聚合物上的附着特性。在离子能量大于10 keV、剂量大于3 × 10 ^ions/cm ^2的条件下，细胞对聚苯乙烯和硅橡胶的离子注入区表现出选择性贴附。<15>另一方面，他们没有在聚酰亚胺上显示出来。在5 keV的低能量条件下，神经细胞表现出选择性附着在聚酰亚胺植入区域。从细胞体的神经突也表现出选择性的延伸超过PS，SR和PI以及细胞附着性能的植入区域。（2）聚合物表面润湿性和蛋白质吸附的表面控制 ...更多信息 用气泡法和空气中水滴法测量了水在离子注入聚合物表面的接触角。我们发现，植入的表面需要在水和培养基中数小时以稳定其表面状态。因此，气泡法是评价细胞粘附性能的合适方法。表面接触角的减小，即，通过碳负离子注入，聚苯乙烯和硅橡胶分别获得了约10 °和20 °的亲水性。另一方面，聚酰亚胺显示接触角增加，即，疏水性，通过植入约15 °。在浸入蛋白质-PBS溶液后，还测试了神经生长因子NGF、FGF和明胶的蛋白质吸附。XPS分析表明，注入C的PS和SR表面的氮含量（来自蛋白质）远高于未注入C的表面。这表明植入C的PS和SR能够吸附蛋白质，从而导致神经细胞和轴突的选择性附着。（3）选择性贴附于PS植入C区的细胞对PS膜下导电耦合电极板施加电脉冲的反应：我们研究了贴附细胞在导电耦合电极板施加电脉冲下的神经突起生长。结果显示，细胞突起优先向正脉冲电极生长，而非向另一电极生长。这意味着人工控制细胞与轴突之间的连接模式的可能性。用斑块法证实了神经细胞的兴奋是由具有电感耦合的外加电脉冲引起的。因此，生物神经系统和外部半导体电路之间的生物界面，能够通过使用我们开发的负离子注入方法形成。少

项目成果

炭素負イオンビームにおる生分解性ポリ乳酸表面の神経細胞親和性の改質

碳负离子束修饰可生物降解聚乳酸表面的神经元亲和力

• 发表时间: 2002
• 期刊: 第1回21世紀連合シンポジウム-科学技術と人間-論文集
• 作者: 辻 博司;泉川雅芳;宇田川善行;佐藤弘子;後藤康仁;石川順三
• 通讯作者: 石川順三

Extracellular Matrix Absorption Properties of Negative Ion-Implanted Polystyrene, Polydimethylsiloxane and Poly-Lactic Acid

负离子注入聚苯乙烯、聚二甲基硅氧烷和聚乳酸的细胞外基质吸收性能

• 发表时间: 2002
• 期刊: Surface and Coatings Technology 158/159
• 作者: H.Tsuji;H.Sasaki;Y.Utsumi;H.Sato;Y.Gotoh;J.Ishikawa
• 通讯作者: J.Ishikawa

辻 博司, 泉川雅芳, 宇田川善行, 佐藤弘子, 後藤康仁, 石川順三: "炭素負イオンビームによる生分解性ポリ乳酸表面の神経細胞親和性の改質"第1回21世紀連合シンポジウム-科学技術と人間-論文集. 393-396 (2002)

Hiroshi Tsuji、Masayoshi Izumikawa、Yoshiyuki Udakawa、Hiroko Sato、Yasuhito Goto、Junzo Ishikawa：“碳负离子束对可生物降解聚乳酸表面神经元亲和力的修饰”第一届21世纪联盟研讨会-科学技术与人类-论文集。 393-396 (2002)

炭素負イオン注入により改質した生分解性ポリ乳酸表面の神経細胞接着特性

碳负离子注入修饰可生物降解聚乳酸表面的神经细胞粘附性能

• 发表时间: 2002
• 期刊: 真空 45
• 作者: 辻 博司;泉川雅芳;佐藤弘子;佐々木仁志;後藤康仁;石川順三
• 通讯作者: 石川順三

Selective Neurite Outgrowth on Silver Negative Ion (Ag)-Implanted Polystyrene Surfaces

植入银负离子 (Ag) 的聚苯乙烯表面选择性神经突生长

• 发表时间: 2004
• 期刊: Chinese Journal of Polymer Science 22
• 作者: H.Sato;H.Tsuji;G.Sasaki;S.Ikemura;Y.Gotoh;J.Ishikawa;S.Nishimoto
• 通讯作者: S.Nishimoto