Structure and Function of Peroxins Essential for Peroxisome Assembly

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Pex14p is a central component of the peroxisomal matrix protein import machinery. We successfully delineated the crystal structure at 1.8 A resolution of the conserved domain comprising the amino-acid residues at 25-70 of mammalian Pex14p. A hydrophobic surface is composed of the conserved residues, including the protruding Phe35 and Phe52, and forms two binding pockets for biding the helical WXXXF/Y motif of Pex5p. As to peroxisomal membrane assembly, we demonstrated that Pex16p functions as the Pex3p-docking site and serves as the peroxisomal membrane receptor that is specific to the Pex3p-Pex19p complexes, termed the class II pathway.