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Structural and functional study of enzymes which are related to central nervous system disorders with muscle deficiency

Structural and functional study of enzymes which are related to central nervous system disorders with muscle deficiency
与肌肉缺乏的中枢神经系统疾病相关的酶的结构和功能研究
批准号:
20370053
负责人:
KATO Ryuichi
金额:
$12.73万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010

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中文摘要
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英文摘要
We elucidated PomT1 and T2 require sugar modification on their proteins for the activity. We also showed that mechanism of PomT1-T2 complex formation is different between human and yeast but similar between human and zebra-fish. Over expression and purification of PomGnT1 protein for X-ray structural determination was successful by wheat germ cell free expression system. Over expression and purification of fukutin and FKRP proteins were also successful by E. coli expression system.
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会议论文
ヒトO-マンノース転移酵素におけるN型糖鎖修飾の解析
人 O-甘露糖基转移酶 N-聚糖修饰分析
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [萬谷博, 赤阪-萬谷啓子, 遠藤玉夫]
通讯作者: 遠藤玉夫
Role on N-glycans on protein O-mannosyltransferases POMT1 and POMT2.
N-聚糖对蛋白质 O-甘露糖基转移酶 POMT1 和 POMT2 的作用。
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [Hiroshi Manya, Keiko Akasaka-Manya, Tamao Endo]
通讯作者: Tamao Endo
DOI: 10.1093/jb/mvp170
发表时间: 2010-03-01
期刊: JOURNAL OF BIOCHEMISTRY
影响因子: 2.7
作者: [Manya, Hiroshi, Akasaka-Manya, Keiko, Endo, Tamao]
通讯作者: Endo, Tamao
構造生物学研究センター報告
结构生物学研究中心报告
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [Hiroshi Matsutaka, Takeru Okada, Yuichi Inoue, Hajime Fukuoka, Shahbaz Khan, Shige H.Yoshimura, Akihiko Ishijima, 加藤龍一]
通讯作者: 加藤龍一
15
    Crystallography of membrane protein complex by S-SAD method
    Establishment of high sensitive Salmonella tester strains expressing mammalian acetvltransferase and sulfotransferase
    • 批准号:
      05557120
    • 项目类别:
      Grant-in-Aid for Developmental Scientific Research (B)
    • 资助金额:
      $9.86万
    • 财政年份:
      1993
    • 负责人:
      KATO Ryuichi
    • 依托单位:
    Analysis of human interindividual and racial differeness of drug response and metabolism : Approach by the method of diagnosis by gene analysis
    • 批准号:
      05454153
    • 项目类别:
      Grant-in-Aid for General Scientific Research (B)
    • 资助金额:
      $4.29万
    • 财政年份:
      1993
    • 负责人:
      KATO Ryuichi
    • 依托单位:
    Assessment of human drug metabolism by expression of cytochrome P-450 in yeasts.
    • 批准号:
      03557113
    • 项目类别:
      Grant-in-Aid for Developmental Scientific Research (B)
    • 资助金额:
      $9.73万
    • 财政年份:
      1991
    • 负责人:
      KATO Ryuichi
    • 依托单位:
    海外基金