Production of human liver cytochrome P-450 by using a biotechnological method and development of the clinical application

生物技术方法生产人肝细胞色素P-450及其临床应用进展

• 批准号: 60870095
• 负责人: KATO Ryuichi
• 金额: $ 6.27万
• 依托单位: School of Medicine, Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870095/
• 关键词: cytochrome P-450; cloning; drug metabolism; クローニング; 遺伝子クローニング

Cytochrome P-450 plays an important role in detoxification of drug and other xenobiotics. To develop the medical use of the enzyme, we have investigated the structures of human liver cytochrome P-450 which provide the basis for the production by a genetic engineering methodology informationf of a major human liver cytochrome P-450 was obtained from <lambda> gtll pharge cDNA livrary by the use of the specific antibodies.

细胞色素P-450在药物和其他外源性物质的解毒过程中起着重要作用。为了开发该酶的医学用途，我们研究了人肝细胞色素P-450的结构，这为通过基因工程方法学生产提供了基础。通过使用特异性抗体从gtll pharge cDNA livrary中获得了主要的人肝细胞色素P-450<lambda>。

项目成果

Kawano, S., Kamataki, T., Yasumori, T., Yamazoe, Y. and Kato, R.: "Purification of human liver cytochrome P-450 catalyzing testosterone 6 <beta> -hydroxylation." J. Biochem.

Kawano, S.、Kamataki, T.、Yasumori, T.、Yamazoe, Y. 和 Kato, R.：“催化睾酮 6 β-羟基化的人肝细胞色素 P-450 的纯化”。

Yasumori, T., Kawano, S., Nagata, K., Shimada, M., Yamazoe, Y. and Kato, R.: "Nucleotide sequence of a human liver cytochrome P-450 related to the rat male specific form." J. Biol. Chem.

Yasumori, T.、Kawano, S.、Nagata, K.、Shimada, M.、Yamazoe, Y. 和 Kato, R.：“与大鼠雄性特定形式相关的人肝细胞色素 P-450 的核苷酸序列。”