Molecular mechanism of development and regulation of sex-specific cytochrome P-450 in rat liver

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Molecular mechanism of development and regulation of sex-specific cytochrome P-450 in rat liver was studied.Sex-specific cytochrome P-450, P-450-male and P-450-female are developmentally regulated. Androgen stimulates the expression of P-450-male and suppresses the expression of P-450-male and estrogen strongly suppresses the expression of P-450-male.Hypophysectomy decreases P-450-male but completely suppresses P-450-female in male rats and expresses P-450-male. Moreover, growth hormone expresses or supresses P-450-male dependent upon the mode of administration. P-450-male is decreased in male rats treated with morphine, reserpine or alloxan and P-450-male is expressed in female rats by the same treatments. The change of testosterone 16 <alpha> -hydroxylation is closely correlated to that of P-450-male, thus testosterone is considered as an endogenous substrate of P-450-male.In addition, benzo(a)pyrene hydroxylase is correlated with P-450-male. Regulation of two new sex-specific P-450, which are responsible for testosterone 6 <beta> -hydroxylation, P-450-6 <beta> - <I> and P-450-6 <beta> - <II> was also studied.