Studies on the structure and function of the nucleus in senescent cells

衰老细胞细胞核结构与功能的研究

• 批准号: 20370079
• 负责人: ISHIKAWA Fuyuki
• 金额: $ 8.65万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20370079/
• 关键词: テロメア; 細胞老化; NF-kappaB; ヘテロクロマチン; ヒストンH1; HMGA; lamin; NFkappaB

We have previously reported that histone H1 disappears and HMGA proteins are increased in cellular senescent cells. In this study, we further examined the structure and function of the nucleus in senescent cells. We found that 1) histone H1 is likely degraded via the ubiqutin-proteasome pathway, 2) the NF-kB pathway is involved in inducing the characteristic gene expression profiles in senescent cells, and 3) the nuclear membrane is structurally and biochemically dysfunctional. These results will provide a means to control the senescence phenotypes.

我们以前曾报道，组蛋白H1消失，HMGA蛋白增加细胞衰老细胞。在本研究中，我们进一步研究了衰老细胞核的结构和功能。我们发现1）组蛋白H1可能通过泛素-蛋白酶体途径降解，2）NF-κ B途径参与诱导衰老细胞中的特征基因表达谱，3）核膜在结构和生化上功能失调。这些结果将提供一种手段来控制衰老表型。

项目成果

Interaction between DNMTl and DNA replication reactions in the SV40 in vitro replication system.

SV40体外复制系统中DNMT1和DNA复制反应之间的相互作用。

• 发表时间: 2008
• 期刊: Cancer science 99
• 作者: Shimamura;S.
• 通讯作者: S.

RPA-like Mammalian Ctc1-Stn1-Ten1 Complex Binds to Single-Stranded DNA and Protects Telomeres Independently of the Pot1 Pathway

• DOI: 10.1016/j.molcel.2009.08.009
• 发表时间: 2009-10-23
• 期刊: MOLECULAR CELL
• 影响因子: 16
• 作者: Miyake, Yasuyuki;Nakamura, Mirai;Ishikawa, Fuyuki
• 通讯作者: Ishikawa, Fuyuki

xTRF2 is required for telomere replication in Xenopus egg extracts.

xTRF2 是非洲爪蟾卵提取物中端粒复制所必需的。

• 发表时间: 2009
• 作者: Nakamura;M.;Nabetani;A.;Mizuno;T.;Hanaoka;F.;Ishikawa;F.;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa;石川冬木;石川冬木;石川冬木;石川冬木;Fuyuki Ishikawa
• 通讯作者: Fuyuki Ishikawa

Low-Dose Stress Responses

低剂量应激反应

• 发表时间: 2009
• 作者: Nakamura;M.;Nabetani;A.;Mizuno;T.;Hanaoka;F.;Ishikawa;F.;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa;石川冬木;石川冬木;石川冬木;石川冬木;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa
• 通讯作者: Fuyuki Ishikawa

老化のメカニズム : 分子生物学の立場から

衰老机制：从分子生物学的角度

• 发表时间: 2008
• 作者: Nabetani;A.;Fuyuki Ishikawa;Fuyuki Ishikawa;Fuyuki Ishikawa;石川冬木
• 通讯作者: 石川冬木