Analysis of molecular mechanism, origin and diversity of gametophytic self-incompatibility systems

配子体自交不亲和系统的分子机制、起源和多样性分析

• 批准号: 20380003
• 负责人: SASSA Hidenori
• 金额: $ 12.15万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380003/
• 关键词: 遺伝子; タンパク質; 自家不和合性; 雌ずい; 花粉; RNase; バラ科; ナス科; Rnase

Analysis of candidates for pollen-determinant in Japanese pear and apple of Rosaceae suggested that multiple and related F-box genes, SFBB, are collaboratively detoxify non-self pistil determinant, S-RNase. Isolation and functional analysis of HT-B gene, a non-S-specific self-incompatibility factor, of petunia showed that, unlike other solanaceous species, even a dramatic suppression of HT-B transcript resulted in partial breakdown of self-incompatibility in petunia. This may suggest slight functional divergence of HT-B gene in different species of Solanaceae.

对蔷薇科日本梨和苹果花粉决定因子候选基因的分析表明，多个相关的F-box基因SFBB协同解毒非自交雌蕊决定因子S-RNase。牵牛花非s特异性自交不亲和因子HT-B基因的分离和功能分析表明，与其他茄属植物不同，即使对HT-B转录本的抑制也会导致牵牛花自交不亲和的部分破坏。这可能提示茄科不同种间HT-B基因存在轻微的功能差异。

项目成果

Identification and functional analysis of non-S-specific pistil-part self-incompatibility factor HT-B of Petunia

矮牵牛雌蕊部非S特异性自交不亲和因子HT-B的鉴定及功能分析

• 发表时间: 2009
• 作者: Puerta AR;Ushijima K;Koba T;Sassa H.
• 通讯作者: Sassa H.

Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus x domestica Borkh.)

• DOI: 10.1007/s11032-008-9249-4
• 发表时间: 2009-04-01
• 期刊: MOLECULAR BREEDING
• 影响因子: 3.1
• 作者: Kim, Hoytaek;Kakui, Hiroyuki;Sassa, Hidenori
• 通讯作者: Sassa, Hidenori

S-RNase遺伝子のCAPS分析によるリンゴ品種のS対立遺伝子22種類の識別

S-RNase基因CAPS分析鉴定苹果品种22S等位基因

• 发表时间: 2008
• 作者: Kim HT;角井宏行;古藤田信博;木庭卓人;佐々英徳
• 通讯作者: 佐々英徳

ニホンナシにおける雌ずい側自家和合性変異ハプロタイプS4smの花粉側特異性の解析

日本梨雌蕊侧自交亲和突变体单倍型S4sm花粉侧特异性分析

• 发表时间: 2010
• 作者: Hiroyuki Kakui;Masaki Kato;Koichiro Ushijima;Miyoko Kitaguchi;Shu Kato;Hidenori Sassa;角井宏行;角井宏行
• 通讯作者: 角井宏行

Sequence divergence and loss-of-function phenotypes of S locus F-box brothers genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia)

• DOI: 10.1111/j.1365-313x.2011.04752.x
• 发表时间: 2011-12-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Kakui, Hiroyuki;Kato, Masaki;Sassa, Hidenori
• 通讯作者: Sassa, Hidenori