权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Functional genomics approach toward identification of pollen-part factor of self-incompatibility in the rosaceous fruit trees.

功能基因组学方法鉴定玫瑰果树自交不亲和的花粉部分因素。

基本信息

批准号：
13660011
负责人：
SASSA Hidenori
金额：
$ 2.3万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660011/
关键词：
self-incompatibility pistil pollen RNase F-box protein Rosaceae ubiquitin almond 自家不和合成コスミドナシ

项目摘要

Pollen-pistil interaction in self-incompatibility (Sl) represents a self-recognition mechanism of flowering plants that lack immune system. The goal of this research is to clarifying the molecular basis of Sl. Sl has been genetically explained by a single S locus with multiple alleles. However, recent findings indicate that Sl reaction of pistil and pollen are controlled by tightly linked different genes, pistil-S and pollen-S genes, respectively. In the three families, Solanaceae, Rosaceae and Scrophulariaceae, the pistil-S gene product is a ribonuclease, S-RNase. Identity of pollen-S gene has long been unknown, and the central issue of this research area.In this study, the S-locus region of a rosaceous fruit tree almond (Prunus dulcis) was extensively characterized to identify pollen-S gene. Through cloning and sequencing of a the S-locus region, a novel pollen-expressed F-box protein gene with haplotype-specific polymorphism was identified. The gene was named SFB for S-haplotype specific F-box protein gene. The characteristics of SFB, proximal location to the S-RNase gene, high level of sequence diversity among alleles, and pollen-specific expression pattern, suggest that it is the first candidate for the pollen-S gene of the S-RNase-based Sl system. The F-box protein is known as a component of SCF complex, a class of E3 ubiquitin ligase. The finding of a new F-box protein gene as the first candidate of pollen-S gene implicates that ubiquitin/proteasome pathway of protein degradation plays a central role in self-incompatibility reaction.

自交不亲和性（Sl）中的花粉-雌蕊相互作用代表了缺乏免疫系统的开花植物的自我识别机制。本研究的目的是阐明 Sl 的分子基础。 Sl 已通过具有多个等位基因的单个 S 基因座进行遗传学解释。然而，最近的研究结果表明，雌蕊和花粉的Sl反应分别由紧密连锁的不同基因——雌蕊-S和花粉-S基因控制。在茄科、蔷薇科和玄参科这三个科中，雌蕊-S基因产物是核糖核酸酶S-RNase。花粉-S基因的身份长期以来一直未知，也是该研究领域的中心问题。本研究对蔷薇科果树杏仁（Prunus dulcis）的S位点区域进行了广泛的表征，以鉴定花粉-S基因。通过对S位点区域的克隆和测序，鉴定出一个新的花粉表达的具有单倍型特异性多态性的F-box蛋白基因。该基因被命名为 SFB，即 S 单倍型特异性 F-box 蛋白基因。 SFB 的特征、与 S-RNase 基因的邻近位置、等位基因之间的高水平序列多样性以及花粉特异性表达模式，表明它是基于 S-RNase 的 Sl 系统的花粉-S 基因的第一个候选者。 F-box 蛋白被认为是 SCF 复合物的组成部分，SCF 复合物是一类 E3 泛素连接酶。作为花粉-S基因的第一个候选基因的新F-box蛋白基因的发现表明蛋白质降解的泛素/蛋白酶体途径在自交不亲和反应中发挥着核心作用。