Functional genomics approach toward identification of pollen-part factor of self-incompatibility in the rosaceous fruit trees.

功能基因组学方法鉴定玫瑰果树自交不亲和的花粉部分因素。

基本信息

批准号：
13660011
负责人：
SASSA Hidenori
金额：
$ 2.3万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660011/
关键词：
self-incompatibility pistil pollen RNase F-box protein Rosaceae ubiquitin almond 自家不和合成コスミドナシ

项目摘要

Pollen-pistil interaction in self-incompatibility (Sl) represents a self-recognition mechanism of flowering plants that lack immune system. The goal of this research is to clarifying the molecular basis of Sl. Sl has been genetically explained by a single S locus with multiple alleles. However, recent findings indicate that Sl reaction of pistil and pollen are controlled by tightly linked different genes, pistil-S and pollen-S genes, respectively. In the three families, Solanaceae, Rosaceae and Scrophulariaceae, the pistil-S gene product is a ribonuclease, S-RNase. Identity of pollen-S gene has long been unknown, and the central issue of this research area.In this study, the S-locus region of a rosaceous fruit tree almond (Prunus dulcis) was extensively characterized to identify pollen-S gene. Through cloning and sequencing of a the S-locus region, a novel pollen-expressed F-box protein gene with haplotype-specific polymorphism was identified. The gene was named SFB for S-haplotype specific F-box protein gene. The characteristics of SFB, proximal location to the S-RNase gene, high level of sequence diversity among alleles, and pollen-specific expression pattern, suggest that it is the first candidate for the pollen-S gene of the S-RNase-based Sl system. The F-box protein is known as a component of SCF complex, a class of E3 ubiquitin ligase. The finding of a new F-box protein gene as the first candidate of pollen-S gene implicates that ubiquitin/proteasome pathway of protein degradation plays a central role in self-incompatibility reaction.

自我兼容性（SL）中的花粉 - - 杆子相互作用代表了缺乏免疫系统的开花植物的自我识别机制。这项研究的目的是阐明SL的分子基础。 SL已通过一个带有多个等位基因的单个S位点来遗传解释。然而，最近的发现表明，雌蕊和花粉的SL反应分别由紧密连接的不同基因，雌蕊-S和花粉-S基因控制。在这三个家族中，茄科，性腺科和舒张科，雌蕊基因产物是核糖核酸酶S-RNase。长期以来，花粉基因的身份一直未知，并且该研究领域的核心问题。在这项研究中，玫瑰花果树杏仁（Prunus dulcis）的S-cocus区域被广泛特征以鉴定花粉基因。通过对S-Ocus区域的克隆和测序，鉴定了一种新型花粉表达的F-box蛋白基因具有单倍型特异性多态性。该基因称为S-Haplotype特异性F-box蛋白基因的SFB。 SFB的特征，S-RNase基因的近端位置，等位基因之间的高序列多样性以及花粉特异性的表达模式，这表明它是基于S-RNase SL SLS系统的花粉S基因的第一个候选者。 F-box蛋白被称为SCF复合物的组成部分，SCF复合物是一类E3泛素连接酶。新的F-box蛋白基因作为花粉基因的第一个候选者的发现暗示蛋白质降解的泛素/蛋白酶体途径在自不兼容反应中起着核心作用。