Establishment of embryonic stem cell lines from in vitro matured and in vitro fertilized dog oocytes and their application to regenerative veterinary medicine

本研究的目的是从体外成熟和受精的狗卵母细胞中建立用于再生兽医学的胚胎干细胞系，并获得了以下结果。(1)我们成功地提高了卵母细胞的生长和成熟，通过控制诱导可育发情在未成熟和间情母犬。(2)采用胶原蛋白凝胶包埋培养法提高犬卵母细胞的体外成熟率。(3)通过培养从子宫角中的囊胚收集的内细胞团形成犬ES样细胞集落，并保持其增殖和形态超过10代。(2)神经元可以简单地从灵长类ES细胞诱导。(3)骨髓基质干细胞移植对脊髓损伤犬的功能恢复无明显影响，应用犬ES细胞治疗脊髓损伤犬尚需进一步研究。

The objective of this study was to establish embryonic stem(ES) cell lines from in vitro matured and fertilized dog oocytes for regenerative veterinary medicine, and the following results were obtained.(1) We successfully enhanced oocyte growth and maturation by controlling the induction of fertile oestrus in immature and dioestrous bitches.(2) The in vitro maturation rate of canine oocytes was improved by embedding and culturing in collagen gels.(3) Canine ES-like cell colonies were formed by culturing inner cell masses collected from blastocysts in uterine horns, and maintained their proliferation and morphology beyond passage 10.(2) Neurons could be induced simply from primate ES cells.(3) Bone marrow stromal cell transplantation caused no complications and could have beneficial effects on functional recovery of dogs with spinal cord injury, and further studies are required to apply canine ES cells for the treatment of these dogs.