Development of cell therapy using induced pluripotent stem cells for dogs with spinal cord injury

本研究的目的是利用诱导多能干细胞（iPS）对脊髓损伤犬进行细胞治疗，并获得以下结果。(1)利用含有4种转录因子的慢病毒，成功地将犬胚成纤维细胞重编程为ips样细胞。犬ips样细胞菌落表现为小鼠胚胎干（ES）细胞样形态，在体外分化为多种细胞类型，并在传代25次后保持其增殖和小鼠ES样形态。(2)犬ips样细胞可诱导神经元。(3)骨髓基质细胞移植无并发症，对脊髓损伤犬的功能恢复有有益作用，应用ips样细胞治疗脊髓损伤犬还需进一步研究。

The objective of this study was to development of cell therapy using induced pluripotent stem(iPS) cells for dogs with spinal cord injury, and the following results were obtained.(1) We successfully reprogrammed canine embryonic fibroblasts into iPS-like cells using lentiviruses containing 4 transcription factors. Canine iPS-like cell colonies displayed a mouse embryonic stem(ES) cell-like morphology, differentiated to multiple cell types in vitro, and maintained their proliferation and mouse ES-like morphology beyond passage 25.(2) Neurons could be induced from canine iPS-like cells.(3) Bone marrow stromal cell transplantation caused no complications and could have beneficial effects on functional recovery of dogs with spinal cord injury, and further studies are required to apply iPS-like cells for the treatment of these dogs.