Pursuit of a new system for a high throughput screening of drugs to develop a new strategy of prevention and treatment of ATL

追求高通量药物筛选新系统，制定ATL防治新策略

• 批准号: 20390267
• 负责人: WATANABE Toshiki
• 金额: $ 12.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390267/
• 关键词: ATL; 発現解析; 薬効評価; トキシコゲノミクス; ハイスループット; イマチニブ; セファランチン; miRNA

We prepared a gene panel of 12 genes that show abnormal expression levels on ATL cells based on the expression profile analyses. The expression levels of these genes were measured by QuantiGene Plex simultaneously and in a short time. The measured values were transformed into an "ATL score" by calculation base on a formula. "ATL score" was used to monitor effects of drugs against ATL cells and cell lines derived from them, although experiments using this system were limited because of the cost. In the process of establishing this system, we obtained detailed information about the expression levels of specific genes in PBMCs of HTLV-1 carriers and ATL patients, which provided information about the changes in expression levels in the course of transformation of HTLV-1-infected cells. Conventional analyses were done on some chemicals as to the effects on the ATL cell lines, which provided interesting information about the ATL-specific activities of these molecules.

基于表达谱分析，我们制备了一个包含12个ATL细胞异常表达水平基因的基因面板。同时用QuantiGene Plex在短时间内检测这些基因的表达水平。根据公式计算，将测量值转化为“ATL评分”。“ATL评分”用于监测药物对ATL细胞及其衍生细胞系的影响，尽管由于成本原因，使用该系统的实验受到限制。在该系统的建立过程中，我们获得了HTLV-1携带者和ATL患者外周血特异性基因表达水平的详细信息，为HTLV-1感染细胞转化过程中表达水平的变化提供了信息。对一些化学物质对ATL细胞系的影响进行了常规分析，为这些分子的ATL特异性活性提供了有趣的信息。

项目成果

Potential Biomarker and Risk-Indicator Genes of ATL in Gene Expression Profiling of HTLV-1 Carriers

HTLV-1 携带者基因表达谱中 ATL 的潜在生物标志物和风险指示基因

• 发表时间: 2009
• 作者: 中野和民;松原亜以子;宇都宮與;山口一成;内丸薫;渡邉俊樹
• 通讯作者: 渡邉俊樹

Chronic / smoldering ATL : long-term follow-up and prognostic factor analysis

慢性/阴燃性ATL：长期随访和预后因素分析

• 发表时间: 2010
• 作者: Takasaki Y;Iwanaga M;Imaizumi Y;Tawara M;Joh T;Kohno T;Yamada Y;Kamihira S;Ikeda S;Miyazaki Y;Tomonaga M;Tsukasaki K
• 通讯作者: Tsukasaki K

ATLリスク・インァィケーター遺伝子同定の試みと、ATL発症における機能解析

尝试鉴定ATL风险指示基因并在ATL发病中进行功能分析

• 发表时间: 2010
• 作者: 中野和民;松原亜以子;矢持忠徳;宇都宮與;山口一成;内丸薫;渡邉俊樹
• 通讯作者: 渡邉俊樹

Over-expression of dominant-nagative Helios isoforms in adult T-cell leukemia (ATL) cells

成人 T 细胞白血病 (ATL) 细胞中显性失活 Helios 亚型的过度表达

• 发表时间: 2010
• 作者: 浅沼里実;中野和民;山岸誠;小川誠司;山口一成;宇都宮與;渡邉俊樹
• 通讯作者: 渡邉俊樹

Loss of miR-31 links NF-.B activation in Adult T-cell leukemia

成人 T 细胞白血病中 miR-31 的缺失与 NF-.B 的激活有关

• 发表时间: 2010
• 作者: 山岸誠;中野和民;三宅在子;加賀美弥生;包明久;宇都宮與;山口一成;浅沼里実;渡邉俊樹
• 通讯作者: 渡邉俊樹