Identification of neural induction domain in stem cells and neuronal regenerative medicine using the peptide derived from the domain

使用源自该结构域的肽鉴定干细胞中的神经诱导结构域和神经元再生医学

• 批准号: 20390389
• 负责人: KANNO Hiroshi
• 金额: $ 12.23万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390389/
• 关键词: 神経再生; 幹細胞; 神経分化ペプチド; 脊髄再生; パーキンソン病; VHL; 機能性ペプチド; 多能性幹細胞; 神経再生医療; 神経幹細胞; 皮膚由来幹細胞; 脊髄損傷; 皮膚幹細胞

We identified a neural induction domain for somatic stem cells at elonign BC binding site in the VHL protein, and show neuronal differentiation of the cells by transfer of the domain peptide linked to protein transduction domain (PTD). In addition, we show that the domain has the same function for the other somatic stem cells, and that BC-box motifs within SOCS-box proteins also have an ability to induce neuronal differentiation. Furthermore, when the domain peptide-transferred stem cells are grafted into recipient nervous tissue, the grafted cells differentiate to neurons and recovery of the neuronal symptom is recognized. Thus, the neuronal differentiation of pluripotent somatic stem cells is occurred by transfer of the neural induction domain peptide linked to PTD and would contribute to neuronal regenerative therapy.

我们在VHL蛋白中的elonign BC结合位点处鉴定了体干细胞的神经诱导结构域，并通过转移连接至蛋白转导结构域（PTD）的结构域肽来显示细胞的神经元分化。此外，我们表明，该结构域具有相同的功能，为其他体干细胞，和SOCS盒蛋白内的BC盒基序也有能力诱导神经元分化。此外，当将转移了结构域肽的干细胞移植到受体神经组织中时，移植的细胞分化为神经元，并且识别出神经元症状的恢复。因此，通过转移与PTD连接的神经诱导结构域肽发生多能成体干细胞的神经元分化，并将有助于神经元再生治疗。

项目成果

Unique multipotent cells in adult human mesenchymal cell populations

• DOI: 10.1073/pnas.0911647107
• 发表时间: 2010-05-11
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Kuroda, Yasumasa;Kitada, Masaaki;Dezawa, Mari
• 通讯作者: Dezawa, Mari

脳神経外科領域における再生医療の展望

再生医学在神经外科领域的前景

• 发表时间: 2009
• 期刊: Current Insights in Neurological Scienece 17(1)
• 作者: 菅野 洋;川原信隆
• 通讯作者: 川原信隆

Practical induction system for dopamine-producing cells from bone marrow stromal cells using spermine-pullulan- mediated reverse transfection method.

使用精胺-普鲁兰多糖介导的反向转染方法从骨髓基质细胞产生多巴胺细胞的实用诱导系统。

• 发表时间: 2009
• 期刊: Tissue Eng Part A 15(7)
• 作者: Nagane K;Kitada M;et al (+3 people)
• 通讯作者: et al (+3 people)

Neuronal differentiation of stem cells by transfer of VHL peptide and regenerative therapy.

通过 VHL 肽转移和再生疗法进行干细胞神经元分化。

• 发表时间: 2010
• 作者: Kanno H;Kubo A;Higashida T;Kawahara N
• 通讯作者: Kawahara N

Committed neural progenitor cells derived from genetically modified bone marrow stromal cells ameliorate deficits in a rat model of stroke

• DOI: 10.1038/jcbfm.2009.62
• 发表时间: 2009-08-01
• 期刊: JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
• 影响因子: 6.3
• 作者: Hayase, Makoto;Kitada, Masaaki;Dezawa, Mari
• 通讯作者: Dezawa, Mari