Isolation of pluriptent somatic stem cells for neuronal regenerative medicine and neuronal differentiation peptide-mediated neuronal differentiation

用于神经元再生医学和神经元分化的多体干细胞的分离肽介导的神经元分化

• 批准号: 23390353
• 负责人: KANNO Hiroshi
• 金额: $ 11.98万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390353/
• 关键词: 多能性組織幹細胞; 神経分化誘導; 神経分化ペプチド; 神経分化ドメイン; モーターニューロン; ドーパミンニューロン; 神経再生医療; BCボックス蛋白; 多能性幹細胞; 機能性ペプチド; BCボックスモチーフ; 神経分化

We used neural stem cells and skin-derived mesenchymal stem cells as pluripotent somatic stem cells to neuronal lineage. Eleven BC-box proteins(SOCS1-7, ASB3, WSB2, LRR1, VHL)-derived functional peptides are delivered to those somatic stem cells. Each peptide induced to differentiate different type of neuron. VHL and socs7-derived peptides induced to differentiate dopaminergic and motor neurons, and SOCS5-derived peptide induced to differentiate retinal color epithelial cells and gultamate neuron. This mechanism of neuronal differentiation is suggested to be related to degeradation of Stat3, but is under investigation.

我们利用神经干细胞和皮肤来源的间充质干细胞作为多能成体干细胞向神经元谱系分化。将11种BC盒蛋白（SOCS 1 -7、ASB 3、WSB 2、LRR 1、VHL）衍生的功能肽递送至这些体干细胞。每种肽诱导分化不同类型的神经元。VHL和SOCS 7衍生肽诱导多巴胺能和运动神经元分化，SOCS 5衍生肽诱导视网膜颜色上皮细胞和谷氨酸神经元分化。这种神经元分化的机制被认为与Stat 3的退化有关，但正在调查中。

项目成果

Role of the von Hippel-Lindau tumor suppressor protein during neuronal differentiation of somatic stem cells and its application to neuronal regeneration

von Hippel-Lindau抑癌蛋白在成体干细胞神经元分化过程中的作用及其在神经元再生中的应用

• 发表时间: 2014
• 期刊: J Transl Med Epidemiol
• 作者: Kanno H;Kubo A;Higashida T
• 通讯作者: Higashida T

Regenerative therapy for neuronal diseases with transplantation of somatic stem cells.

• DOI: 10.4252/wjsc.v5.i4.163
• 发表时间: 2013-10-26
• 期刊: World journal of stem cells
• 影响因子: 4.1
• 作者: Kanno, Hiroshi

Role of the von Hippel-Lindau tumor suppressor protein in neuronal differentiation of somatic stem cells and its application to neuronal regeneration: a review

von Hippel-Lindau肿瘤抑制蛋白在成体干细胞神经元分化中的作用及其在神经元再生中的应用：综述

• 发表时间: 2014
• 期刊: Journal of Translational Medecine & Epidemiology
• 作者: Kanno H;Higashida T;Kubo A
• 通讯作者: Kubo A

The VHL tumor suppressor protein regulates tumorigenicity of U87-derived glioma stem-like cells by inhibiting the JAK/STAT signaling pathway

• DOI: 10.3892/ijo.2013.1773
• 发表时间: 2013-03-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Kanno, Hiroshi;Sato, Hidemitsu;Yamada, Sachiko
• 通讯作者: Yamada, Sachiko

VHL regulates tumorigenecity of cancer stem cells through in hibition of Stat3 signaling pathways

VHL 通过抑制 Stat3 信号通路调节癌症干细胞的致瘤性

• 发表时间: 2012
• 作者: Kanno H;Yoshizumi T;Yamada S
• 通讯作者: Yamada S