THE ESTABLISHMENT OF JAPAN-ORIGINAL GENE THERAPY BY ANTI-TUMOR ANGIOGENESIS USING DECOY GENE.

日本原创的利用诱饵基因抗肿瘤血管生成的基因疗法的建立。

• 批准号: 20390478
• 负责人: ISHIBASHI Hiroaki
• 金额: $ 12.06万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390478/
• 关键词: 実験腫瘍学; 血管新生; 悪性新生物; 遺伝子治療; 口腔がん; 口腔癌

The conditioned media(CM) harvested from human pulmonary squamous cell carcinoma(QG56), pulmonary small cell carcinoma(QG90) and gastric adenocarcinoma(MKN28) cultivated under hypoxic conditions(3% O2), enhanced the angiogenic activity in vitro more than those obtained under normoxic cultivation(20% O2). The total length of the tube structures formed by bovine capillary endothelial cells(BCEs) in the CM cultured at 3% O2 was about 1. 5(QG56 and MKN28) or 1. 9(QG90) times longer than that at 20% O2. Tube formation was diminished by the preincubation of CM with anti-basic fibroblast growth factor(bFGF) IgG. After performing the fractionations of the CM and the crude extracts of cell lysates cultured using a heparin-Sepharose column, the mitogenic activity in the CM from all cancer cells at 3% O2 was about 2 times higher than that in the CM at 20% O2, while it decreased in the cell lysates at 3% O2 to about 40% of those at 20% O2. This mitogenic activity of BCEs in the CM from all cancer cells was almost totally suppressed by anti-basic fibroblast growth factor(bFGF) IgG, but not with anti-vascular endothelial growth factor(VEGF) IgG. These results suggest that the hypoxic condition is an important cause for tumor angiogenesis by bFGF or bFGF-like molecule(s) derived from tumor cells.

从人肺鳞状细胞癌（QG56）、肺小细胞癌（QG90）和胃腺癌（MKN28）中获得的条件培养基（CM）在缺氧条件下（3% O2）培养，比在常氧培养（20% O2）下获得的条件培养基（CM）在体外增强血管生成活性。牛毛细血管内皮细胞（BCEs）在3% O2培养的CM中形成的管状结构总长度约为1。5（QG56和MKN28）或1。9（QG90）比20% O2时长。用抗碱性成纤维细胞生长因子（bFGF） IgG预孵育CM可减少管的形成。用肝素- sepharose色谱柱对CM和细胞裂解物粗提物进行分离后，所有癌细胞在3% O2下的CM中有丝分裂活性比在20% O2下的CM高2倍左右，而在3% O2下的细胞裂解物中有丝分裂活性下降到20% O2下的40%左右。抗碱性成纤维细胞生长因子（bFGF） IgG几乎完全抑制所有癌细胞CM中bce的有丝分裂活性，而抗血管内皮生长因子（VEGF） IgG则不具有抑制作用。这些结果提示，缺氧条件是肿瘤细胞衍生的bFGF或bFGF样分子生成肿瘤血管的重要原因。

项目成果

Our approach for early detection of oral cancer using smear cytology

我们使用涂片细胞学早期检测口腔癌的方法

• 发表时间: 2010
• 期刊: Korean Association of Oral and Maxillofacial Surgeons
• 作者: Kikuchi M;Ishibashi H;et al.
• 通讯作者: et al.

Oral symptoms including dental erosion in gastroesophageal reflux disease are associated with decreased salivary flow volume and swallowing function

• DOI: 10.1007/s00535-011-0515-6
• 发表时间: 2012-04-01
• 期刊: JOURNAL OF GASTROENTEROLOGY
• 影响因子: 6.3
• 作者: Yoshikawa, Hiroo;Furuta, Kenji;Sekine, Joji
• 通讯作者: Sekine, Joji

Management of tongue squamous cell carcinoma in pregnant women : Report of two Patients

孕妇舌鳞状细胞癌的治疗：两名患者的报告

• 发表时间: 2009
• 期刊: Asian J Oral Maxillofac Surg
• 作者: Kondo S;Ishibashi H;Nariai Y;Kato T;Enomoto Y;Karino M;Sekine J
• 通讯作者: Sekine J

A fish bone in the tongue: A report of a case

舌头里有鱼刺：一例报告

• DOI: 10.1016/j.ajoms.2009.03.001
• 发表时间: 2010
• 期刊: Asian Journal of Oral and Maxillofacial Surgery
• 作者: Y. Nariai;C. Yanai;S. Kondo;H. Ishibashi;Masayoshi Egawa;H. Kitagaki;J. Sekine
• 通讯作者: J. Sekine

Study on feasibility of cervical lymphnode metastasis in squamous cell carcinoma.

鳞状细胞癌颈部淋巴结转移的可行性研究。

• 发表时间: 2008
• 期刊: Proc Int Oral Cancer 3
• 作者: Karino M.;Ishibashi H.;et al.
• 通讯作者: et al.