THE DEVELOPMENT OF NEWLY JAPAN-ORIGINAL GENE THERAPY FOR DEGENERATIVE ARTHROSIS USING DECOY GENE.

使用培养的细胞检测几种细胞因子刺激的uPA和MMPs的表达。用TNFα刺激培养的TMJ软骨细胞，可增加uPA和MMPs的表达。检测与细胞因子刺激相关的转录因子。TNFα刺激软骨组织来源的培养细胞可激活转录因子NF-κ B和Sp1。基因转染条件的建立我们采用HVJ-脂质体法建立了靶向NF-κ B和Sp1的诱饵寡核苷酸的转染条件，常规转染率为100%。通过Decoy ODNs转染抑制uPA和MMPs的表达通过将Decoy ODNs转染至来自软骨组织的培养细胞中抑制uPA和MMPs的表达。

Examination for the expression of uPA and MMPs stimulated by several cytokines using cultured cells. The expression of uPA and MMPs was increased by the stimulation with TNFαusing cultured cells derived from cartilaginous tissue of TMJ. Examination of transcriptional factor related to cytokines stimulation. Transcriptional factors, NF-kB and Sp1, were activated by TNFαstimulation at the cultured cells derived from cartilaginous tissue. Establishment of the condition for the gene transfer We established the condition for the Decoy oligodeoxynucletides targeted to NF-kB and Sp1 using HVJ-liposome method, and decoy ODNs were routinely transferred to 100% of the cells. Inhibition of the expression of uPA and MMPs by Decoy ODNs transfection The expression of the uPA and MMPs were inhibited by the transfection of Decoy ODNs to the cultured cells derived from cartilaginous tissue.