THE DEVELOPMENT OF NEWLY JAPAN-ORIGINAL GENE THERAPY FOR DEGENERATIVE ARTHROSIS USING DECOY GENE.

使用诱饵基因开发新的日本原创基因疗法治疗退行性关节炎。

• 批准号: 21659433
• 负责人: ISHIBASHI Hiroaki
• 金额: $ 2.09万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659433/
• 关键词: 免疫; 感染; 炎症; 細胞外基質; 顎関節; 遺伝子治療; 顎関節症; 基質破壊酵素; 遣伝子治療

Examination for the expression of uPA and MMPs stimulated by several cytokines using cultured cells. The expression of uPA and MMPs was increased by the stimulation with TNFαusing cultured cells derived from cartilaginous tissue of TMJ. Examination of transcriptional factor related to cytokines stimulation. Transcriptional factors, NF-kB and Sp1, were activated by TNFαstimulation at the cultured cells derived from cartilaginous tissue. Establishment of the condition for the gene transfer We established the condition for the Decoy oligodeoxynucletides targeted to NF-kB and Sp1 using HVJ-liposome method, and decoy ODNs were routinely transferred to 100% of the cells. Inhibition of the expression of uPA and MMPs by Decoy ODNs transfection The expression of the uPA and MMPs were inhibited by the transfection of Decoy ODNs to the cultured cells derived from cartilaginous tissue.

用培养细胞检测几种细胞因子刺激下uPA和MMPs的表达。TMJ软骨组织培养细胞经tnf α刺激后，uPA和MMPs的表达增加。检测与细胞因子刺激相关的转录因子。tnf α刺激软骨组织培养细胞后，转录因子NF-kB和Sp1被激活。利用hvj -脂质体法建立了靶向NF-kB和Sp1的诱骗寡脱氧核苷酸的条件，并将诱骗寡脱氧核苷酸常规转移到100%的细胞中。转染诱饵ODNs对软骨组织培养细胞中uPA和MMPs表达的抑制作用

项目成果

Our approach for early detection of oral cancer using smear cytology

我们使用涂片细胞学早期检测口腔癌的方法

• 发表时间: 2010
• 期刊: Korean Association of Oral and Maxillofacial Surgeons
• 作者: Kikuchi M;Ishibashi H;et al.
• 通讯作者: et al.

Oral symptoms including dental erosion in gastroesophageal reflux disease are associated with decreased salivary flow volume and swallowing function

• DOI: 10.1007/s00535-011-0515-6
• 发表时间: 2012-04-01
• 期刊: JOURNAL OF GASTROENTEROLOGY
• 影响因子: 6.3
• 作者: Yoshikawa, Hiroo;Furuta, Kenji;Sekine, Joji
• 通讯作者: Sekine, Joji

Management of tongue squamous cell carcinoma in pregnant women : Report of two Patients

孕妇舌鳞状细胞癌的治疗：两名患者的报告

• 发表时间: 2009
• 期刊: Asian J Oral Maxillofac Surg
• 作者: Kondo S;Ishibashi H;Nariai Y;Kato T;Enomoto Y;Karino M;Sekine J
• 通讯作者: Sekine J

Newly gene therapy for temporomandibular joint arthritis by in vitro cis-transelement decoy against TNFα-induced NF-kB activation

通过体外顺反元件诱饵对抗 TNFα 诱导的 NF-kB 激活来治疗颞下颌关节关节炎的新基因疗法

• 发表时间: 2011
• 作者: Ishibashi H;et al.
• 通讯作者: et al.

A fish bone in the tongue

舌头里有鱼刺

• 发表时间: 2010
• 期刊: Asian J Oral Maxillofac Surg
• 作者: Nariai Y;Yanai C;Kondo S;Ishibashi H;et al.
• 通讯作者: et al.