Novel methods of regeneration of tooth using bone marrow cells-aim at order medical treatment-

点击翻译按钮获取中文摘要

Several cell lines derived from periodontal membrane, dental pulp, inner enamel epithelium and bone marrow of BALB/cA mouse were established. When the enamel epithelial cells were cultured in the growth medium supplemented with the products of human dental pulp cells, the enamel epithelial cells produced ameloblastin. The pellets of ameroblastin producing cells and dental pulp cells were inserted into the collagen gel adjoining each other. Then these constructions were enveloped with human periodontal membrane. Lastly, they were enveloped with collagen sponge and cultured GM supplemented with the ETFs by circumfusion apparatus.We established 27 tissue stem cell lines derived from human bone marrow. Three of them made teratoma when they were transplanted into the subcutis of nude mouse. The stem cells were cultured by hanging drop method with ETFs, the embryoid bodies were developed. The embryoid bodies were brought up with ETFs to the embryo-like structures by circumfusion apparatus. However, there is nothing of the tooth germ structures yet.