Development of the instrument for measuring respiration of cancer cells with oxygen electrode as a metablic indicator for anticancer drug screening

评估药物对癌细胞作用的新趋势是测量其诱导的代谢活动，而传统的方法采用群体生长法，如组织培养药物反应法（HDRA）或(CD-DST)-MTT法。我们将呼吸量作为癌细胞代谢的指标，开发了廉价的一次性氧电极传感器和多通道溶解氧仪（DOX-10和DOX-96）。使用这些仪器，细胞毒性测定15小时，该方法具有一步测定简便，对自动化系统适应性强等优点。该氧电极方法在抗肿瘤药物敏感性试验和动物实验替代中的总体可用性进行了评价。

New trend in methods for assessing pharmacological action to cancer cells is to mesure their metabolic activities induced, while the conventional methods used population growth, such as histoculture drug responseassay, (HDRA) or (CD-DST)-MTT assay, method. We focused n respiration volume as an indicato of cancer cell metabolism, and developed inexpensive disposable oxygen electrode sensor and multi-channnel dissolved oxygen meters (DOX-10 and DOX-96). Using these instruments, cytotoxicity was measured for 15 hours and the method showed superior features to conventional methods in its handiness of one step assay, and excellent adaptability to automated systems.Total usability of this oxygen electrode method is being evaluated in anticancer drug susceptibility test and alternative to animal experiment.