Development of the instrument for measuring respiration of cancer cells with oxygen electrode as a metablic indicator for anticancer drug screening

氧电极测量癌细胞呼吸作为抗癌药物筛选代谢指标的仪器研制

• 批准号: 09557097
• 负责人: ISHIKAWA Hiroshi
• 金额: $ 7.17万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557097/
• 关键词: oxygen electrode; anticancer drug susceptibility; cancer cell respiration; dissolved oxygen measuring system; chemotherapy; 酸素電極; 癌薬剤感受性; MTT法; 迅速測定システム; 化学療法; ネオアジュバント療法; 癌薬剤感受性試験; 溶存酸素電極; 抗癌剤選択システム; マイトマイシンC; HL-60細胞; HTMM細胞; TPA

New trend in methods for assessing pharmacological action to cancer cells is to mesure their metabolic activities induced, while the conventional methods used population growth, such as histoculture drug responseassay, (HDRA) or (CD-DST)-MTT assay, method. We focused n respiration volume as an indicato of cancer cell metabolism, and developed inexpensive disposable oxygen electrode sensor and multi-channnel dissolved oxygen meters (DOX-10 and DOX-96). Using these instruments, cytotoxicity was measured for 15 hours and the method showed superior features to conventional methods in its handiness of one step assay, and excellent adaptability to automated systems.Total usability of this oxygen electrode method is being evaluated in anticancer drug susceptibility test and alternative to animal experiment.

评价肿瘤细胞药理作用的新趋势是测定其诱导的代谢活性，而传统的方法是采用群体生长的方法，如组织培养药物反应法(HDRA)或(CD-DST)-MTT法。我们将n呼吸量作为癌细胞代谢的指标，并开发了廉价的一次性氧电极传感器和多通道溶解氧仪(DOX-10和DOX-96)。利用该仪器进行了15h的细胞毒性测定，该方法具有一步法简便、对自动化系统适应性强等优点，正在抗癌药物敏感性试验和动物实验的替代试验中进行评价。

项目成果

新井潤一郎: "汎用溶存酸素測定装置による抗がん剤薬剤感受性試験方法" Human Cell. 11(3). 175-178 (1998)

Junichiro Arai：“使用通用溶解氧测量装置的抗癌药物敏感性测试方法”Human Cell 11(3) (1998)。

Yasuda M: "Evaluation of a new method for an in vitro susceptibility test of anticancer drugs(ACD) using a new type of oxygen electrodes."International Gynecologic Cancer Society. 493-497 (1999)

安田 M：“使用新型氧电极评估抗癌药物体外药敏试验 (ACD) 的新方法。”国际妇科癌症协会。

Amano Yoshihisa: "Measuring respiration of cultured cell with oxygen electrode as a metabolic indicator for drug screening"Human Cell. 12(1). 3-10 (1999)

天野义久：“用氧电极测量培养细胞的呼吸作为药物筛选的代谢指标”《人体细胞》。

Senwa Unten: "Anticancer drug susceptibility test of the cancer by oxygen electrode method."Human Cell. 11(2). 49 (1998)

Senwa Unten：“通过氧电极法进行癌症的抗癌药物敏感性测试。”人体细胞。

Makoto Yasuda: "Evaluation of a new method for an in vitro susceptibility test of anticancer drugs (ACD) using a new type of oxygen electrodes."International Gynecologic Cancer Society. 493-497 (1999)

Makoto Yasuda：“使用新型氧电极评估抗癌药物体外药敏试验 (ACD) 的新方法。”国际妇科癌症协会。