Expression of bioactivity through nano-sized defects and its regulation

纳米缺陷表达生物活性及其调控

• 批准号: 20500413
• 负责人: AIZAWA Mamoru
• 金额: $ 2.08万
• 依托单位: Meiji University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500413/
• 关键词: 水酸アパタイト; 欠陥構造; 透過型電子顕微鏡; 骨芽細胞; 生体活性; 多孔質セラミックス; 骨伝導(生体活性); 骨誘導; 電子顕微鏡; バイオセラミックス; アパタイト; 生体材料; セラミックス; 細胞・組織; ナノ構造

Biological apatite presented in bone and teeth of mammals contains various minerals, and has lots of defects with nano-scale sizes in the crystal structure. In the present study, we fabricated hydroxyapatite ceramics including bone minerals (bone HAp ceramics) as model materials to clarify the relationship between nano-defect structure and bioactivity of biological apatite. The results of high-resolution transmission electron microscope (HR-TEM) revealed that bone HAp ceramics contained more defects and strains compared to pure HAp ceramics. In addition, osteoblasts derived from rat bone marrow cells were seeded on bone HAp ceramics, together with pure HAp ceramics as a control. The bone HAp ceramics promoted the differentiation into osteoblast compared to pure HAp ceramics. The present bone HAp ceramics may be expected as next-generation artificial bone graft.

哺乳动物骨骼和牙齿中存在的生物磷灰石含有多种矿物质，晶体结构中存在大量纳米级尺寸的缺陷。在本研究中，我们制备了包含骨矿物质的羟基磷灰石陶瓷（骨HAp陶瓷）作为模型材料，以阐明纳米缺陷结构与生物磷灰石生物活性之间的关系。高分辨率透射电子显微镜（HR-TEM）结果显示，与纯HAp陶瓷相比，骨HAp陶瓷含有更多的缺陷和应变。此外，将源自大鼠骨髓细胞的成骨细胞接种在骨HAp陶瓷上，并与纯HAp陶瓷一起作为对照。与纯HAp陶瓷相比，骨HAp陶瓷促进了成​​骨细胞的分化。目前的骨HAp陶瓷有望成为下一代人工骨移植物。

项目成果

クサビラオレンジブタ脛骨埋入による高強度化アパタイトファイバースキャフォルドの生体適合性評価

库萨平橙猪胫骨植入高强度磷灰石纤维支架的生物相容性评价

• 发表时间: 2011
• 作者: 永井亜希子;et al.;中原広道,李相男,柴田攻;鴈本拓也・島田愛生・安冨由美子・本田みちよ・水本みのり・松成ひとみ・竹内靖浩・長嶋比呂志・相澤守
• 通讯作者: 鴈本拓也・島田愛生・安冨由美子・本田みちよ・水本みのり・松成ひとみ・竹内靖浩・長嶋比呂志・相澤守

Development of Apatite-fiber Scaffolds for Tissue Engineering and Their Application to Bone and Liver Regenerations

组织工程磷灰石纤维支架的开发及其在骨和肝脏再生中的应用

• 发表时间: 2010
• 作者: Masugata H;Senda S;Goda F;et al.;浅沼俊彦;Mamoru Aizawa
• 通讯作者: Mamoru Aizawa

In vivo bone formation using three-dimensional scaffolds developed from a single crystal apatite fiber.

使用由单晶磷灰石纤维开发的三维支架进行体内骨形成。

• 发表时间: 2009
• 期刊: J.Biomed.Mater.Res.A 90A
• 作者: H.Morisue;M.Matsumoto;M.Aizawa;et al.
• 通讯作者: et al.

Cell proliferation, morphology and differentiation of Transgenic-cloned pig calvarial osteoblasts on the silicon-substituted hydorxyapatite ceramics fabricated viaultrasonic spray-pyrolysis technique

超声喷雾热解硅取代羟基磷灰石陶瓷上转基因克隆猪颅骨成骨细胞的细胞增殖、形态和分化

• 发表时间: 2011
• 期刊: J.Aust.Ceram.Soc 47
• 作者: M.Honda;K.Kikushima;T.Konishi;M.Mizumoto;H.Matsunari;H.Nagashima;M.Aizawa
• 通讯作者: M.Aizawa

Fabrication of chelate-setting cements from silicon-substituted apatite powder prepared by ultrasonic spray-pyrolysis technique and its property

超声喷雾热解硅取代磷灰石粉体螯合水泥的制备及其性能

• 发表时间: 2009
• 期刊: Archives of BioCeramics Research 9
• 作者: K.Kikushima;K.Oribe;M.Aizawa
• 通讯作者: M.Aizawa