Development of method for accurate measurement of energy expenditure in a short-time period with whole body indirect human calorimeter

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For accurate measurement of energy expenditure (EE) in a short-time period with whole body indirect human calorimeter (HC), we developed a gas infusion equipment simulates CO2 pattern dynamically exchanges in the HC. Accuracy of CO2 volume infused with the equipment was validated using catalog accuracies of components of the equipment. Repeated gas infusion experiments suggested that the necessity of 1) the estimation of real (not the catalog accuracy) CO2 volume infused with the equipment, 2) the adjustment of flow rate conversion factor of the equipment, 3) the development of software for simple operation inputting the infusion flow rate data to personal computer.