Characterization and biological assessment of DNA-Protein crosslink repair in vertebrate cells

为了阐明DNA-蛋白质交联(DPC)修复的过程，我们采用了鸡DT40细胞的反向遗传方法。有研究表明，内源性活性氧参与了DPC的形成。我们克隆了几个ROS清除酶基因敲除细胞，以阐明抗氧化防御系统的机制。此外，我们还试图在小鼠水平上寻找DPC产生的化学物质HMTA的治疗潜力。到目前为止，所有的结果都已经发表在同行评议的期刊上。

To elucidate the process of DNA-Protein Crosslink (DPC) repair, we employed reverse genetic approach using chicken DT40 cells. It has been suggested that endogenous reactive oxygen species (ROS) is involved in formation of DPC. We generated several ROS scavenge enzyme genes knock out cells to elucidate mechanism of antioxidant defense system. In addition, we try to look for the therapeutic potential of DPC producing chemical, HMTA, in mouse level. All the results so far, already published in peer reviewed journals.