Molecular breeding of transgenic tree for phytoremediation of aromatic compounds

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Many white-rod fungi produce many lignin peroxidases. Among them, Mnp2 of Pleurotus ostreatus was reported to degrade recalcitrant polyaromatic compounds because of its strong peroxidase activity. We introduced a fusion gene encoding poplar cellulase signal sequence and mnp2 mature region under the control of leaf specific or root specific promoter into Arabidopsis thaliana. Several transgenic plants showed the ability to degrade BisphenolA. This suggests the possibility of molecular breeding of transgenic tree which can express the mnp2 gene. We also introduced a chlorocatechol dioxigenase gene of a soil bacterium Ralstonia eutropha NH9 under the control of CaMV35S promoter into poplar plant (hybrid aspen) and this gene was successfully expressed in the transgenic poplar plant.