Synthesis of ss-glucan oligosaccharides and their biological evaluation

在这份报告中，我们描述了β （1，3）中含有氨基烷基组及其生物评估的合成和生物评估。A2，3 diol glycoside与a4，6 benziliden作为β ·1，3）链接glycoside合成物的有效糖基受体。使用线性四乙酰和分支五乙酰乙酰作为允许的糖基供体β ·1，3）线性八分之六和分支的非子-用于在良好的全字段中准备的组合。对寡藻糖基化合物对溶解性结构的结合--1对固体支持的精神分裂症（SPG）的竞争性影响的衡量表明，分支的七氟醚糖基化合物和线性六氟醚糖基化合物2也有对该学科的结合活动。在补充中，两个寡核苷酸在荧光素辅助的NF-kB试验中被禁止了一项反活性活动。这代表了由Dectin-1介导的纯合成寡藻糖苷刺激免疫的第一个演示。

In this report, we describe the synthesis and biological evaluation of β (1,3) oligosaccharides possessing an aminoalkyl group and their biological evaluation. A 2,3 diol glycoside with a 4,6 benziliden protecting group finctioned as an effective glycosyl acceptor for the synthesis ofβ ・1,3)linked glycosides. Using a combination of a linear tetrasaccharide and a branched pentasaccharide as glycosyl donors permitted β ・1,3)linear octa-to hexadecasaccharides and branched nona- to heptadecasaccharides to be prepared in good total yields. Measurements of the competitive effects of oligosaccharides on the binding of a soluble dectin-1 to a solid-supported Schizophyllan (SPG) revealed that the branched heptadecasacccharide and the linear hexadecasacccharides 2 also have binding activity for to dectine-1. In addition, the two oligosaccharides exhibited agonist activity in a luciferase-assisted NF-kB assay. This represents the first demonstration of a purely synthetic oligosaccahrides stimulating immunity mediated by dectin-1.