Functional analysis of Streptomyces prolyl oligopeptidase family

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The aim of present study was to reveal enzymatic properties of three types belonged to a family of prolyl oligopeptidase in Streptomyces genomes. The evidence presented in this study demonstrates that one is an exo-aminopeptidase, and the other is an endo-peptidase. The later shows an trypsin-like activity, and is belonged to a family of oligopeptidase B. Furthermore, by substitution of the active serine residue in the aminopeptidase with cysteine, its aminolysis activity is more enhanced than that of the wild-type enzyme.