Development of tailor-made catalyst (catalytic antibody) by heterologous immunization

点击翻译按钮获取中文摘要

In this study we have studied the improvement of the catalytic antibodies by newly devised heterologous immunization. We have developed a method to improve the molecular recognition of the catalytic antibody in antigen-combining site. In heterologous immunization, in which acetyl-transition-state analog was injected into mice after injection of trifluoroacetyl-transition-state analog, catalytic antibodies with higher hydrolytic activities were obtained. The kinetic parameters and amino acid sequences were determined to discuss the catalytic mechanism of the catalytic antibodies.