Generation of MicroAntibody by directed evolution

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In this study, we have developed a method to downsize the antibody by the combination of structure-design of proteins and directed evolution. We have constructed a library of alternative-binding peptide with non-immunoglobulin domain to develop molecular probe and molecular targeting drug. The helix-loop-helix peptide, which binds to specifically target molecules, is named as "MicroAntibody". We have constructed a library of MicroAntibody to generate human IgG-Fc-specific peptides and Aurora kinase-specific peptides. We also studied immunogenicity and stability of MicroAntibody