Studies on the introduction of transition-metal compounds into the antigen-combining site of tailor-made artificial enzyme (catalytic antibody)

在这项研究中，我们开发了将过渡金属化合物作为辅因子引入抗原结合部位以发挥金属酶功能的方法。由于过渡金属催化了多种化学转化，在抗原结合部位引入过渡金属拓宽了抗体催化剂的通用性。我们研究了两种将过渡金属引入抗原结合部位的方法：(1)免疫含有过渡金属化合物的过渡态类似物；(2)利用全酶技术将含有人工辅因子的过渡金属引入抗原结合部位。

In this study we have developed methods for the introduction of transition metal compounds as cofactors into the antigen-combining site to function as metal enzymes. Because transition metal catalysis catalyzes a variety of chemical transformations, the introduction of a transition metal into the antigen-combining site widen the versatility of antibody catalysts. We have studied two methods to introduce the transition metal to the antigen-combining site, (1) immunization of the transition-state analog which contains transition metal compounds, and (2) introduction of transition metal containing artificial cofactors into the antigen-combining site by using the technique of holoabzyme.