Molecular mechanism of cyanobacterial circadian clock using the reconstituted in-vitro oscillator.

在三磷酸腺苷存在的情况下，生物钟可以在体外由KaIA、Kaib和KaiC蛋白重建。本研究对蓝藻中被认为是氧化还原感受器的LdpA进行了生化分析。氧化还原态改变了分子结构。此外，还发现KaiABC重组系统的pH值对周期的长度有影响。细胞内pH和LdpA这两个因素可能参与了光引起的周期长度的变化。

The circadian clock can be reconstituted in vitro by the KaiA, KaiB, and KaiC proteins in the presence of ATP. In this study, LdpA presumed to be a redox sensor in cyanobacteria was analyzed biochemically. The structure of the molecule was changed by a redox state. Moreover, it was found that pH of the KaiABC reconstitution system have an effect on the length of the cycle. The two factors, pH in the cell and LdpA, could get involved in the change of the period length the cycle by light.