Functional Analysis of Arabidopsis AtRBR1 after sucrose starvation

蔗糖饥饿后拟南芥AtRBR1的功能分析

• 批准号: 20570042
• 负责人: SEKINE Masami
• 金额: $ 3.16万
• 依托单位: Ishikawa Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570042/
• 关键词: 細胞周期; シロイヌナズナ; 栄養飢餓; RNAi

Although sucrose availability is crucial for commitment to plant cell division during G1 phase by controlling the expression of D-type cyclins, it has remained unclear how these factors mediate entry into the cell cycle. Here we show that Arabidopsis RETINOBLASTOMA-RELATED PROTEIN 1 (AtRBR1) is involved in G1-phase cell cycle arrest caused by sucrose starvation. We generated estrogen-inducible AtRBR1 RNA interference (RNAi) Arabidopsis suspension MM2d cells, and found that downregulation of AtRBR1 leads to a higher frequency of arrest in G2 phase, instead of G1-phase arrest in the uninduced control, after sucrose starvation. Downregulation of AtRBR1 also stimulated the activation of E2F-regulated genes when these genes were repressed in the uninduced cells under the limited sucrose conditions. We conclude that AtRBR1 is a key effector for the ability of sucrose to modulate progression from G1 phase.

虽然蔗糖的可用性是至关重要的承诺，植物细胞分裂在G1期通过控制D型细胞周期蛋白的表达，它仍然不清楚这些因素如何介导进入细胞周期。在这里，我们表明，拟南芥视网膜母细胞瘤相关蛋白1（AtRBR1）参与G1期细胞周期停滞引起的蔗糖饥饿。我们构建了雌激素诱导的AtRBR1 RNA干扰（RNAi）拟南芥悬浮细胞MM2d，发现AtRBR1的下调导致蔗糖饥饿后G2期阻滞的频率更高，而非未诱导对照的G1期阻滞。AtRBR1的下调也刺激激活E2F调节的基因时，这些基因在未诱导的细胞在有限的蔗糖条件下被抑制。我们得出结论，AtRBR1是一个关键效应的蔗糖调节从G1期进展的能力。

项目成果

RETINOBLASTOMA-RELATED PROTEIN 1 is involved in G1 phase cellcycle arrest caused by sucrose starvation.

视网膜母细胞瘤相关蛋白 1 参与蔗糖饥饿引起的 G1 期细胞周期停滞。

• 发表时间: 2008
• 期刊: Plant Molecular Biology 66
• 作者: Hirano;H.;Harashima;H.;Sekine;M.;Shinmyo;A.
• 通讯作者: A.

細胞周期停止に関与するAtRBRlの機能解析

AtRBR1参与细胞周期停滞的功能分析

• 发表时间: 2008
• 作者: 中嶋香織;平野博人;新名惇彦;関根政実
• 通讯作者: 関根政実

ホームページ等。

主页等

Both negative and positive G1 cell cycle regulators undergo proteasome-mediated degradation during sucrose starvation in Arabidopsis.

在拟南芥蔗糖饥饿期间，阴性和阳性 G1 细胞周期调节因子都会经历蛋白酶体介导的降解。

• 期刊: Plant Signaling & Behavior in press
• 作者: Hirano;H.;Shinmyo;A.;Sekine;M.
• 通讯作者: M.

Key Arabidopsis cell cycle proteins undergo proteasome-mediated degradation during sucrose starvation.

关键的拟南芥细胞周期蛋白在蔗糖饥饿期间经历蛋白酶体介导的降解。

• 发表时间: 2011
• 期刊: Plant Physiology and Biochemistry 49
• 作者: Hirano;H.;Shinmyo;A.;Sekine;M.
• 通讯作者: M.