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The mechanism controlling the cell cycle and differentiation in plants

植物细胞周期和分化的控制机制

基本信息

批准号：
09640773
负责人：
SEKINE Masami
金额：
$ 1.98万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640773/
关键词：
cyclin Cdc2 cell cycle Rb protein 分化 Rb

项目摘要

Regulation of the cell cycle is highly conserved among eukaryotes, and many of the genes involved have been isolated from wide range of organisms such as yeasts, animals and plants. In mammalian cells, progression through the G1/S transition is controlled by cyclin D in conjunction with their catalytic partners, CDK4 and CDK6. The essential role of cyclin D/CDK complex is to direct phosphorylation of pRb, thereby driving cells through the restriction point at late Gi phase.We have isolated four cDNA clones for cyclin D (NtcycD3), which all belong to the CycD3 class, Using the highly synchronized suspension culture of tobacco BY-2 cells , we analyzed the transcript levels of these genes during the cell cycle. In contrast to mammalian cyclin D, NtcycD3 showed various expression patterns in the cell cycle.Mammalian cyclin D can form active complexes with CDK4 and CDK6, but not with Cdc2. Although a number of cdc2-related and cyclin genes have been isolated in plants, it is not elucidated which combination of these proteins forms active complexes. We have produced NtcycD3 and tobacco Cdc2 (Cdc2Nt1) with a baculovirus expression system and found that these proteins formed a complex. Surprisingly, the complex can phosphorylate the tobacco Rb-related protein in vitro.

细胞周期的调控在真核生物中是高度保守的，并且所涉及的许多基因已经从广泛的生物体如酵母、动物和植物中分离。在哺乳动物细胞中，通过G1/S转换的进展是由细胞周期蛋白D与它们的催化伴侣CDK 4和CDK 6一起控制的。我们分离了4个cyclin D（NtcycD_3）的cDNA克隆，它们都属于CycD_3类，利用烟草BY-2细胞的高度同步悬浮培养，分析了这些基因在细胞周期中的转录水平。与哺乳动物细胞周期蛋白D不同，NtcycD 3在细胞周期中表现出不同的表达模式，哺乳动物细胞周期蛋白D可与CDK 4和CDK 6形成活性复合物，但不与Cdc 2形成活性复合物。虽然一些cdc 2相关基因和细胞周期蛋白基因已经在植物中被分离出来，但这些蛋白质的哪种组合形成活性复合物还没有阐明。我们已经用杆状病毒表达系统产生了NtcycD 3和烟草Cdc 2（Cdc 2Nt 1），并发现这些蛋白形成了复合物。令人惊讶的是，该复合物可以在体外磷酸化烟草Rb相关蛋白。