Sequence-specificity and binding-cooperativity of multiple DNA-binding domains of CUT-homeodomain transcription factors

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The DNA-binding mechanism by multiple DNA-binding domains of CUT-homeodomain transcription factors were analyzed by methods of structural biology and physical chemistry. NMR and isothermal titration calorimetry analyses revealed that CUTr1 domain and homeodomain of transcription factor SATB1 bind to DNA containing TAATA sequence in a cooperative manner. A SELEX experiment showed that CUTr1 has the major role in determining the sequence specificity.