Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka

底板细胞产生 Wnt4b 对于青鳉脊柱的节段模式至关重要

• 批准号: 20570199
• 负责人: INOHAYA Keiji
• 金额: $ 3.16万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570199/
• 关键词: 器官形成; メダカ; 骨形成; 脊椎発生; Wnt; 発生・分化

Analysis of a spontaneous medaka mutant, fused centrum ( fsc), which exhibits fused centra and the absence of the intervertebral ligaments, revealed that fsc encodes wnt4b, which was expressed exclusively in the floor plate. In fsc mutants, wnt4b expression was completely lost in the floor plate and that abnormal conversion of the intervertebral ligament cells into osteoblasts appeared to cause a defect of the intervertebral ligaments. These findings provide a novel perspective on the mechanism of vertebrate development.

对青鳉自发突变体融合中心（fsc）的分析表明，fsc 编码 wnt4b，该突变体表现出融合中心和椎间韧带的缺失，该基因仅在底板中表达。在 fsc 突变体中，底板中 wnt4b 表达完全丧失，椎间韧带细胞异常转化为成骨细胞似乎导致椎间韧带缺陷。这些发现为脊椎动物发育机制提供了新的视角。

项目成果

Bef medaka mutant reveals the essential role of c-myb in both primitive anc definitive hematopoiesis.

Bef 青鳉突变体揭示了 c-myb 在原始造血和最终造血中的重要作用。

• 发表时间: 2010
• 期刊: Developmental Biology. 345
• 作者: Moriyama A;Inohaya K;Maruyama K;Kudc A.
• 通讯作者: Kudc A.

Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka

• DOI: 10.1242/dev.051540
• 发表时间: 2010-06-01
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Inohaya, Keiji;Takano, Yoshiro;Kudo, Akira
• 通讯作者: Kudo, Akira

sec24d encoding a component of COPII is essential for vertebra formation, revealed by the analysis of the medaka mutant, vbi

• DOI: 10.1016/j.ydbio.2010.03.016
• 发表时间: 2010-06-01
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Ohisa, Satoshi;Inohaya, Keiji;Kudo, Akira
• 通讯作者: Kudo, Akira

Bef medaka mutant reveals the essential role of c-myb in both primitive and definitive hematopoiesis

• DOI: 10.1016/j.ydbio.2010.06.031
• 发表时间: 2010-09-15
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Moriyama, Akemi;Inohaya, Keiji;Kudo, Akira
• 通讯作者: Kudo, Akira

Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion

• DOI: 10.1111/j.1742-4658.2008.06722.x
• 发表时间: 2008-12-01
• 期刊: FEBS JOURNAL
• 影响因子: 5.4
• 作者: Sano, Kaori;Inohaya, Keiji;Yasumasu, Shigeki
• 通讯作者: Yasumasu, Shigeki