The visualization of amitosis cells and genetic analysis of somatic hybrids in Citrus species

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Tetraploid culture cells were induced successfully from the long term-subcultured embryogenic calli of Satsuma mandarin (Citrus unshiu Marc.), although it was not possible to observe the amitosis cells. Genetic analysis of the putative triploid somatic hybrid SG1 obtained from electrofusion between Shougun mandarin (C.reticulata Blanc., 2n=2x=18)and L-1 grapefruit (C.paradisi Macf., 2n=2x=18)were investigated. Results of flow cytometry and RAPD analyses suggested that this was a hybrid with the haploid genome of the shougun mandarin. This triploid somatic hybrid SG1 was also the grapefruit with excellent characteristics such as seedless and parthenocarpy.