Molecularbiological study on a novel organomercurial decomposing enzyme from iron-oxidizing bacterium Acidithiobacillus ferrooxidans and use of the enzyme for bioremediation of mercury

铁氧化菌氧化亚铁硫杆菌新型有机汞分解酶的分子生物学研究及其在汞生物修复中的应用

• 批准号: 20580079
• 负责人: SUGIO Tsuyoshi
• 金额: $ 3万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580079/
• 关键词: 鉄酸化細菌; 水銀還元酵素; 有機水銀分解酵素; チトクロームcオキシダーゼ; Acidithiobacillus ferrooxidanas; iron-oxidizing bacterium; mercury; organomercurial; acidithiobacillus ferrooxidans; mercury reduction; mercury volatilization; mercury resistance; cytochrome c oxidase

It has been known that iron-oxidizing bacterium Acidithiobacillus ferrooxidans has a mercuric reductase (MerA) which reduces Hg^<2+> to a volatilizable metal mercury (Hg^0), but not has a mercuric lyase (MerB) which cleaves a C-Hg bond in organomercurials. Author newly found a novel Hg^0 volatilizing enzyme system in a highly mercury resistant A. ferrooxidans MON-1. Namely, strain MON-1 has a mercury resistant aa3-type cytochrome c oxidase ( aa_3) and α- and β-subunits in aa_3 has activities to cleave C-Hg bond in organomercurials and to reduce Hg^<2+> to Hg^0.

已知铁氧化细菌Acidithiobacillusferrooxidans具有汞还原酶（MerA），该还原酶可将Hg^2+还原为可挥发的金属汞（Hg^0），但不具有汞裂解酶（MerB），该酶可裂解有机汞化合物中的C-Hg键。作者在一株高抗汞A. ferrooxidans MON-1。即MON-1菌株具有抗汞的aa_3型细胞色素c氧化酶（aa_3），aa_3中的α-和β-亚基具有裂解有机汞化合物中的C-Hg键和将Hg^2+还原为Hg ^0的活性。

项目成果

Reduction of Hg2+ with Reduced Mammalian Cytochrome c by Cytochrome c Oxidase Purified from a Mercury-Resistant Acidithiobacillus ferrooxidans Strain, MON-1

从耐汞氧化亚铁硫杆菌菌株 MON-1 中纯化的细胞色素 c 氧化酶对 Hg2 进行还原，从而还原哺乳动物细胞色素 c

• 发表时间: 2008
• 期刊: Bioscience, biotechnology and biochemistry
• 作者: T. Sugio;M. Fujii;Y. Ninomiya;T. Kanao;A. Negishi;F. Takeuchi
• 通讯作者: F. Takeuchi

Reduction of cytochrome c by tetrathionate in the presence of tetrathionate hydrolase purified from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells

在从硫生长的氧化亚铁硫杆菌 ATCC 23270 细胞中纯化的连四硫酸盐水解酶存在下，连四硫酸盐还原细胞色素 c

• 发表时间: 2009
• 期刊: Adranced Materials Research Vols 71-73
• 作者: Taha;T.M.;F.Takeuchi;T.Sugio
• 通讯作者: T.Sugio

Mercury Volatilization from Organomercurials by Mercury-Resistant Acidithiobacillus ferrooxidans MON-1

抗汞氧化亚铁硫杆菌 MON-1 导致有机汞中的汞挥发

• 发表时间: 2010
• 作者: SUGIO T.;KOMODA;T.;OKAZAKI;Y.;TAKEDA Y.;NAKAMUR;S.;TAKEUCHI F.
• 通讯作者: TAKEUCHI F.

A.ferrooxidans D3-2株由来亜硫酸酸化酵素系のc型cytochrome及びaa_3型cytochrome oxidaseからの再構成

用 c 型细胞色素和 aa_3 型细胞色素氧化酶重建 A.ferrooxidans D3-2 菌株的亚硫酸盐氧化酶系统

• 发表时间: 2008
• 作者: 杉尾剛;T.Taha;早崎琢也;赤穂亜実;竹内文章
• 通讯作者: 竹内文章

Sulfite Oxidation Catalyzed by aa 3-Type Cytochrome c Oxidase in Acidithiobacillus ferrooxidans

氧化亚铁硫杆菌中aa 3型细胞色素c氧化酶催化的亚硫酸盐氧化

• DOI: 10.1271/bbb.100446
• 发表时间: 2010
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: T. Sugio;Ami Ako;F. Takeuchi
• 通讯作者: F. Takeuchi