The molecular mechanism underlying the co-regulation of plasma membrane and F-actin

质膜与F-肌动蛋白共同调控的分子机制

• 批准号: 20580096
• 负责人: IHARA Sayoko
• 金额: $ 3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580096/
• 关键词: 細胞応答; 細胞骨格; 細胞膜; アクチン; アクチ

Cells change their morphology under various physiological conditions. Co-regulation of plasma membrane and cytoskeleton is required for these changes, the mechanism of which is not fully understood. We addressed the issue by functional analysis of SWAP-70, which binds to both membrane and actin in a stimulus-dependent way. We found that SWAP-70 binds to integrin. 1 in a pull down assay, and the activation of integrin. 1 rescued SWAP-70-deficient phenotype, suggesting that SWAP-70 may function by activating integrin. 1 through their interaction.

细胞在各种生理条件下改变其形态。这些变化需要质膜和细胞骨架的共同调节，其机制尚不完全清楚。我们通过对SWAP-70的功能分析解决了这个问题，SWAP-70以刺激依赖的方式与膜和肌动蛋白结合。我们发现SWAP-70与整合素结合。1，以及整联蛋白的活化。1挽救了SWAP-70缺陷型，表明SWAP-70可能通过激活整合素发挥作用。1、通过互动

项目成果

出芽酵母の前胞子膜伸長に必要なPP1複合体の機能解析

酿酒酵母前孢子膜伸长所需的 PP1 复合物的功能分析

• 发表时间: 2010
• 作者: Mika Sekiya;Ako Hiraishi;Maiko Touyama;Kazuichi Sakamo to;大平孝博
• 通讯作者: 大平孝博

Protein Phosphatase Type 1-Interacting Protein Ysw1 Is Involved in Proper Septin Organization and Prospore Membrane Formation during Sporulation

• DOI: 10.1128/ec.00095-09
• 发表时间: 2009-07-01
• 期刊: EUKARYOTIC CELL
• 作者: Ishihara, Makoto;Suda, Yasuyuki;Tachikawa, Hiroyuki
• 通讯作者: Tachikawa, Hiroyuki

Rac1結合タンパク質DEF6の機能解析

Rac1结合蛋白DEF6的功能分析

• 发表时间: 2008
• 作者: 加勢優子;高原有未;岡努;福井泰久;伊原さよ子
• 通讯作者: 伊原さよ子

A mutant of SWAP-70, a phosphatidylinositoltrisphosphate binding protein, transforms mouse embryo fibroblasts, which is inhibited by sanguinarine.

• DOI: 10.1371/journal.pone.0014180
• 发表时间: 2010-12-02
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Fukui Y;Ihara S
• 通讯作者: Ihara S

Rap1 activation is required for DEF6-mediated cell shape change

DEF6 介导的细胞形状改变需要 Rap1 激活

• 发表时间: 2008
• 作者: Yuko Kase;Tsutomu Oka;Yasuhisa Fukui;Sayoko Ihara
• 通讯作者: Sayoko Ihara