Development of discrimination method between Dojou and Kara-dojou loaches using DNA markers for conservation of rice paddy ecosystem

点击翻译按钮获取中文摘要

Two loaches, the Dojou Misgurnus anguillicaudatus and Kara-dojou Paramisgurnus dabryanus inhabiting in rice paddies may be discriminated each other using mitochondrial and nuclear DNA markers in this study. Applying mitochondrial DNA analysis, the Dojou was separated into four genetic clades, Europe, China, Korea and Japan types. The Kara-dojou formed a single clade, Kara-dojou type. Results of nuclear DNA analysis showed the possibility of no hybridizing individual of them. To use a species discrimination equation with valuables of barbell length and body height could be available to decrease in a number of specimens used in the DNA analysis.