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Development of an isolation method of microsatellite DNA markers for evaluation of genetic diversity of organisms in rural areas

Development of an isolation method of microsatellite DNA markers for evaluation of genetic diversity of organisms in rural areas
农村地区生物遗传多样性微卫星DNA标记分离方法的建立
批准号：
23580340
负责人：
KOIZUMI Noriyuki
金额：
$3.33万
依托单位：
National Agriculture and Food Research Organization
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2011
资助国家：
日本
项目状态：
已结题
起止时间：
2011 至 2013
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580340/
关键词：
マイクロサテライトDNA遺伝子多様性農村生態系遺伝的多様性DNA

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中文摘要
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英文摘要
An isolation method of microsatellite DNA markers was developed for the evaluation of genetic diversity of organisms inhabiting rural areas. This method was composed of five processes such as DNA extraction, DNA fragmentation, screening of microsatellite regions, etc. Applying this method, nineteen to forty of novel microsatellite DNA markers were isolated and characterized for seven species of fish, dragonfly and mysid collected from agricultural canals. Furthermore, these markers were available for practical analysis of their genetic diversity and the structures of their populations. The development of the markers for other species based on this method and the investigation of genetic diversity using the developed markers are expected in the near future.
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Genetic diversity and population structure of the Hotoke loach, Lefua echigonia, a Japanese endangered loach. Ed by Baptista, G. R., An Integrated View of the Molecular Recognition and Toxinology - From Analytical Procedures to Biomedical Applications
日本濒危泥鳅鳅的遗传多样性和种群结构。
DOI： --
发表时间： 2013
期刊：
影响因子： --
作者： [Koizumi, N., Mizutani, M., Watabe, K(他3名)]
通讯作者： K(他3名)
Thirty-two polymorphic microsatellite loci of the mysid crustacean Mesopodopsis tenuipes.
糠虾甲壳动物 Mesopodopsis tenuipes 的 32 个多态性微卫星位点。
DOI： 10.1007/s12686-011-9470-z
发表时间： 2013
期刊： Conservation Genet Resour
影响因子： --
作者： [Koizumi, N., Morioka, S., Mori, A.(他5名), Kiyotada Hayashi, 木全　卓, 渡辺浩二，田中丸治哉，多田明夫, Koizumi N. et al.]
通讯作者： Koizumi N. et al.
Trends and future subjects of research on fish ecology, conservation and management in agricultural canals and ditches.
农业运河沟渠鱼类生态、保护和管理研究的趋势和未来主题。
DOI： 10.3825/ece.15.281
发表时间： 2012
期刊： Ecology and Civil Engineering
影响因子： --
作者： [Koizumi, N., Morioka, S., Quinn, T. W.(他5名), 田中暁大，田中丸治哉，多田明夫, 木全　卓，森　匠平, 武山絵美,九鬼康彰,東口阿希子,奥村啓史, 田中丸治哉，多田明夫, 小出水規行・西田一也・水谷正一]
通讯作者： 小出水規行・西田一也・水谷正一
Development of nineteen novel polymorphic microsatellite loci of Clupeichthys aesarnensis
伊萨十九个新多态性微卫星位点的开发
DOI： 10.1007/s12686-013-9887-7
发表时间： 2013
期刊： Conservation Genetics Resources
影响因子： 1.1
作者： [Koizumi, N., Morioka, S., Mori, A.(他5名)]
通讯作者： A.(他5名)
共 15 条
    Development of discrimination method between Dojou and Kara-dojou loaches using DNA markers for conservation of rice paddy ecosystem
    • 批准号：
      20580270
    • 项目类别：
      Grant-in-Aid for Scientific Research (C)
    • 资助金额：
      $3.0万
    • 财政年份：
      2008
    • 负责人：
      KOIZUMI Noriyuki
    • 依托单位：
      National Agricultural Research Organization
    Elucidation of genetic network and structure among fish populations in agricultural canals
    • 批准号：
      18580250
    • 项目类别：
      Grant-in-Aid for Scientific Research (C)
    • 资助金额：
      $2.3万
    • 财政年份：
      2006
    • 负责人：
      KOIZUMI Noriyuki
    • 依托单位：
      National Agricultural Research Organization
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