Development of an isolation method of microsatellite DNA markers for evaluation of genetic diversity of organisms in rural areas

建立了一种用于评价农村生物遗传多样性的微卫星DNA标记分离方法。该方法由DNA提取、DNA片段化、微卫星区筛选等5个步骤组成。应用该方法，从农业河道中采集的7种鱼类、蜻蜓和蛹蝇中分离出19 ~ 40个新的微卫星DNA标记并进行了鉴定。此外，这些标记可用于实际分析其遗传多样性和群体结构。在此基础上开发其他物种的遗传标记，并利用所开发的标记进行遗传多样性的研究是未来的发展方向。

An isolation method of microsatellite DNA markers was developed for the evaluation of genetic diversity of organisms inhabiting rural areas. This method was composed of five processes such as DNA extraction, DNA fragmentation, screening of microsatellite regions, etc. Applying this method, nineteen to forty of novel microsatellite DNA markers were isolated and characterized for seven species of fish, dragonfly and mysid collected from agricultural canals. Furthermore, these markers were available for practical analysis of their genetic diversity and the structures of their populations. The development of the markers for other species based on this method and the investigation of genetic diversity using the developed markers are expected in the near future.