Development of an isolation method of microsatellite DNA markers for evaluation of genetic diversity of organisms in rural areas

农村地区生物遗传多样性微卫星DNA标记分离方法的建立

• 批准号: 23580340
• 负责人: KOIZUMI Noriyuki
• 金额: $ 3.33万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580340/
• 关键词: マイクロサテライトDNA; 遺伝子多様性; 農村生態系; 遺伝的多様性; DNA

An isolation method of microsatellite DNA markers was developed for the evaluation of genetic diversity of organisms inhabiting rural areas. This method was composed of five processes such as DNA extraction, DNA fragmentation, screening of microsatellite regions, etc. Applying this method, nineteen to forty of novel microsatellite DNA markers were isolated and characterized for seven species of fish, dragonfly and mysid collected from agricultural canals. Furthermore, these markers were available for practical analysis of their genetic diversity and the structures of their populations. The development of the markers for other species based on this method and the investigation of genetic diversity using the developed markers are expected in the near future.

建立了一种用于农村生物遗传多样性评价的微卫星DNA标记分离方法。 该方法由DNA提取、DNA片段化、微卫星区域筛选等5个步骤组成。应用该方法，从7种采集自农业水渠的鱼类、蚯蚓和糠虾中分离到19 ~ 40个新的微卫星DNA标记。 此外，这些标记可用于实际分析其遗传多样性和群体结构。 在不久的将来，基于这种方法的标记的开发和遗传多样性的调查，使用开发的标记是值得期待的。

项目成果

Genetic diversity and population structure of the Hotoke loach, Lefua echigonia, a Japanese endangered loach. Ed by Baptista, G. R., An Integrated View of the Molecular Recognition and Toxinology - From Analytical Procedures to Biomedical Applications

日本濒危泥鳅鳅的遗传多样性和种群结构。

• 发表时间: 2013
• 作者: Koizumi;N.;Mizutani;M.;Watabe;K(他3名)
• 通讯作者: K(他3名)

Thirty-two polymorphic microsatellite loci of the mysid crustacean Mesopodopsis tenuipes.

糠虾甲壳动物 Mesopodopsis tenuipes 的 32 个多态性微卫星位点。

• DOI: 10.1007/s12686-011-9470-z
• 发表时间: 2013
• 期刊: Conservation Genet Resour
• 作者: Koizumi;N.;Morioka;S.;Mori;A.(他5名);Kiyotada Hayashi;木全 卓;渡辺浩二，田中丸治哉，多田明夫;Koizumi N. et al.
• 通讯作者: Koizumi N. et al.

Trends and future subjects of research on fish ecology, conservation and management in agricultural canals and ditches.

农业运河沟渠鱼类生态、保护和管理研究的趋势和未来主题。

• DOI: 10.3825/ece.15.281
• 发表时间: 2012
• 期刊: Ecology and Civil Engineering
• 作者: Koizumi;N.;Morioka;S.;Quinn;T. W.(他5名);田中暁大，田中丸治哉，多田明夫;木全 卓，森 匠平;武山絵美,九鬼康彰,東口阿希子,奥村啓史;田中丸治哉，多田明夫;小出水規行・西田一也・水谷正一
• 通讯作者: 小出水規行・西田一也・水谷正一

Development of nineteen novel polymorphic microsatellite loci of Clupeichthys aesarnensis

伊萨十九个新多态性微卫星位点的开发

• DOI: 10.1007/s12686-013-9887-7
• 发表时间: 2013
• 期刊: Conservation Genetics Resources
• 影响因子: 1.1
• 作者: Koizumi;N.;Morioka;S.;Mori;A.(他5名)
• 通讯作者: A.(他5名)

Origin of the two major distinct mtDNA clades of the Japanese population of the oriental weather loach Misgurnus anguillicaudatus (Teleostei: Cobitidae)

• DOI: 10.25225/fozo.v60.i4.a12.2011
• 发表时间: 2011-12
• 期刊: Folia Zoologica
• 作者: T. Kitagawa;Y. Fujii;N. Koizumi