Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A.

使用组蛋白脱乙酰酶抑制剂和 latrunculin A 提高源自基因修饰的微型猪细胞的核移植胚胎的发育能力。

• 批准号: 20580311
• 负责人: YOSHIDA Mitsutoshi
• 金额: $ 3.08万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580311/
• 关键词: 体細胞クローン; 遺伝子改変; 脱アセチル化; トリコスタチンA; スベロイルビスヒドロキサム酸; スクリパタイド; ラトランキュリンA; クラウン系ミニブタ; 発生; バルプロ酸

The objective of this study was to examine the effects of postactivation treatment of histone deacetylase inhibitors(HADCi) and an actin polymerisation inhibitor, latrunculin A(LatA) on in vitro and in vivo development of somatic cell nuclear transfer(SCNT) embryos derived from gene^modified Clawn miniature pig cells. After electric activation, SCNT embryos were treated with or without LatA, and cultured in vitro. The rate of blastocyst formation was significantly higher(p<0. 05) in SCNT embryos treated with HADCi and 0. 5μM LatA than that in control. In addition, fetuses and piglets were obtained from recipient uteri after transfer of embryos treated with HDACi and LatA. The results of this study showed for the first time that postactivation treatment with HDACi and LatA was effective to improve in vitro and in vivo developmental capacity of gene-modified cloned miniature pig embryos.

本研究的目的是研究组蛋白去乙酰化酶抑制剂（HADCi）和肌动蛋白聚合抑制剂latrunculin A（LatA）的后激活处理对来自基因修饰的克劳小型猪细胞的体细胞核移植（SCNT）胚胎的体外和体内发育的影响。电激活后，SCNT胚胎用或不用LatA处理，并在体外培养。囊胚形成率显著高于对照组（p<0. 05）。05)在用HADCi和0. 5μM LatA。此外，在移植用HDACi和LatA处理的胚胎后，从受体子宫获得胎儿和仔猪。本研究的结果首次表明，HDACi和LatA的后激活处理是有效的，以提高在体外和体内发育能力的基因修饰的克隆小型猪胚胎。

项目成果

Latrunculin A Dramatically Improves the Developmental Capacity of Nuclear Transfer Embryos Derived from Gene-Modified Clawn Miniature Pig Cells

• DOI: 10.1089/cell.2009.0066
• 发表时间: 2010-04-01
• 期刊: CELLULAR REPROGRAMMING
• 影响因子: 1.6
• 作者: Himaki, Takehiro;Mori, Hironori;Yoshida, Mitsutoshi
• 通讯作者: Yoshida, Mitsutoshi

Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results to ward production of xenograft-competent miniature pigs

通过体细胞核移植生产转基因猪囊胚：异种移植能力小型猪的初步结果

• 发表时间: 2010
• 期刊: J Reprod Dev
• 作者: Himaki T;Watanabe S;Chi H;Yoshida M;Miyoshi K;Sato M
• 通讯作者: Sato M

Effects of demecolcine and sucrose on the incidence of cytoplasmic protrusions containing chromosomes in pig oocytes matured in vitro

• DOI: 10.1262/jrd.19142
• 发表时间: 2008-04-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Miyoshi, Kazuchika;Mori, Hironori;Yoshida, Mitsutoshi
• 通讯作者: Yoshida, Mitsutoshi

バルプロ酸がミニブク体細胞クローン胚の体外発生に及ぼす影響

丙戊酸对Minibuku体细胞克隆胚胎体外发育的影响

• 发表时间: 2009
• 作者: Miyoshi K;Inoue S;Himaki T;Mikawa S;Yoshida M;Miyoshi K;Nakayama A;Ohtsuka M;溝部大和;Wang JH;末友雅士;小澤明央;森寛倫;津田健一郎;森寛倫
• 通讯作者: 森寛倫

プロスタグランジンF2α類縁物質と性腺刺激ホルモン投与によるクラウン系雌ミニブタの発情同期化に関する研究

前列腺素F2α类似物和促性腺激素对冠雌性小型猪发情同步化的研究

• 发表时间: 2009
• 期刊: 鹿児島県獣医師会会報
• 作者: 日巻武裕;梅野知子;三好和睦;吉田光敏
• 通讯作者: 吉田光敏