Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A.

点击翻译按钮获取中文摘要

The objective of this study was to examine the effects of postactivation treatment of histone deacetylase inhibitors(HADCi) and an actin polymerisation inhibitor, latrunculin A(LatA) on in vitro and in vivo development of somatic cell nuclear transfer(SCNT) embryos derived from gene^modified Clawn miniature pig cells. After electric activation, SCNT embryos were treated with or without LatA, and cultured in vitro. The rate of blastocyst formation was significantly higher(p<0. 05) in SCNT embryos treated with HADCi and 0. 5μM LatA than that in control. In addition, fetuses and piglets were obtained from recipient uteri after transfer of embryos treated with HDACi and LatA. The results of this study showed for the first time that postactivation treatment with HDACi and LatA was effective to improve in vitro and in vivo developmental capacity of gene-modified cloned miniature pig embryos.