Studies on the ecology of Bartonella species by molecular biological approach

调查了野生鼠类和输入鼠类巴尔通体的流行情况。我们还在啮齿动物和无家可归者的体表寄生虫中检测了巴尔通体基因。从685只鼠类中分离出176只巴尔通体，分离率为25.7%。这些巴尔通体分离株均来自郊区捕获的鼠类（阳性率为51.8%）。在2007-2008年期间，美国33.3%的体虱和25%的头虱感染的无家可归者有金塔纳白蝇的DNA。此外，序列分析表明，在泰国33个恙螨库和1个野生啮齿动物蜱虫库中检测到的DNA与先前从该国3名患者中分离到的塔米伊布氏杆菌序列相似。调查了546例（28种）输入性小兽类巴尔通体的流行情况。共分离巴尔通体407株，检出动物携带人畜共患巴尔通体4株，新型巴尔通体6株。

We investigated the prevalence of Bartonella species in wild and imported rodents. We also detected the genes of Bartonella in ectoparasite collected from rodents and homeless peoples. We successfully isolated Bartonella organisms from 176 of the 685 rodents (isolation rate, 25.7%). Those Bartonella isolates were all obtained from the rodents captured in suburban areas (rate, 51.8%). During 2007-2008, 33.3% of body lice- and 25% of head lice-infested homeless peoples in USA had DNA of B. quintana. Furthermore, sequence analysis demonstrated that DNA detected in 33 chigger mite pools and one tick pool from wild rodents in Thailand was similar to B. tamiae sequences previously isolated from three patients in the country. We investigated the prevalence of Bartonella spp.in 546 imported small mammals (28 species). We obtained 407 Bartonella isolates and the animals examined were found to harbor 4 zoonotic Bartonella spp.and 6 novel Bartonella spp..