Ecology and pathogenicity of Bartonella henselae and the related organisms

汉赛巴尔通体及相关生物的生态学和致病性

• 批准号: 10660307
• 负责人: MARUYAMA Soichi
• 金额: $ 1.86万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660307/
• 关键词: Antibody; Cat; Cat scratch disease; Bartonella henselae; Bartonella clarridgeiae; opidemiology; prevalence; 16S rRNA; 猫ひっかき病; パルスフィールドゲル電気泳動; 間接蛍光抗体法; ウェスタンブロッティング; 猫; 疫学; Toxoplasma gondii

Among 471 cat serum samples collected from 7 veterinary clinics in Kanagawa and Saitama Prefectures, 9.1% (41/471) and 8.7% (41/471) of cats harbored antibodies against Bartonella henselae and Toxoplasma gondii, respectively. B. henselae-positive rate in male cats (12.9%) was significantly higher than that in female (p<0.01). On the other hands, the seropositive rate of T. gondii in male (9.1%) was almost same with that in female. Furthermore, a total of 7.2% (50/690) of cats was infected with Bartonella species (0% in Hokkaido Prefecture 〜 20% in Okinawa Prefecture) and most B. henselae isolates were identified as type I. It was found for the first time that cats in Japan were infected with B. clarridgeiae alone or both B. henselae and B. clarridgeiae. From these results, it is necessary to take the prevention measures of cat scratch disease such as the development of vaccine for cats.Genome patterns and the antigenicities were examined with 2 isolates from the United States and 8 isolates from Japan. Genome patterns were different in each isolate and the size measured 1.75 〜 2.13Mbp. There was no relationship between the genomes patterns and antigenicities. Therefore, the development of suitable diagnosis measure with isolates from Japan is one of significant problems to be solved.

从神奈川县和埼玉县7家兽医诊所采集的471份猫血清样本中，分别有9.1%（41/471）和8.7%（41/471）的猫携带汉氏巴尔通体和弓形虫抗体。雄性猫中B. henselae的阳性率（12.9%）显着高于雌性猫（p<0.01）。另一方面，男性弓形虫血清阳性率(9.1%)与女性几乎相同。此外，共有7.2%（50/690）的猫感染了巴尔通体（北海道县为0%〜冲绳县为20%），大多数B. henselae分离株被鉴定为I型。首次发现日本的猫单独感染B. clarridgeiae或同时感染B. clarridgeiae。从这些结果来看，有必要采取猫抓病的预防措施，例如开发猫用疫苗。对来自美国的2个分离株和来自日本的8个分离株进行了基因组模式和抗原性检查。每个分离株的基因组模式不同，大小为 1.75 〜 2.13Mbp。基因组模式和抗原性之间没有关系。因此，利用日本分离株开发合适的诊断方法是需要解决的重大问题之一。

项目成果

Maruyama, S., Hiraga, S., Yokoyama, E., Naoi, M., Tsuruoka, Y., Ogura, Y., Tamura, K., Namba, S., Kameyama, Y., Nakamura, S., and Katube, Y: "Seroprevalence of Bartonella henselae and Toxoplasma gondii infections among pet cats in Kanagawa and Saitama Pre

丸山 S.、平贺 S.、横山 E.、直井 M.、鹤冈 Y.、小仓 Y.、田村 K.、难波 S.、龟山 Y.、中村 S.、

Maruyama, S., Nakamura, Y., Kabeya, H., Tanaka, S., Sakai, T., and Katsube, Y.: "Prevalence of Bartonella henselae, Bartonella clarridgeiae and the 16S rRN Gene Type of Bartonella henselae among Pet Cats in Japan."J. Vet. Med. Sci. 62. 273-279 (2000)

Maruyama, S.、Nakamura, Y.、Kabeya, H.、Tanaka, S.、Sakai, T. 和 Katsube, Y.：“宠物中汉赛巴尔通体、克拉里奇巴尔通体的流行率以及汉赛巴尔通体的 16S rRN 基因类型

Maruyama, S. and Katsube, Y.: "Contributions of Microbiology, vol.1 Bartonella and Afipia species with special regard to Bartonella henselae."Karger press, Basel, Switzerland.. 217 (1998)

Maruyama, S. 和 Katsube, Y.：“微生物学贡献，第 1 卷巴尔通体和 Afipia 物种，特别是巴尔通体。”Karger 出版社，瑞士巴塞尔。217 (1998)

Maruyama, S., Tanaka, T., Sakai, T., and Katsube, Y.: "Prevalence of Bartonella species among pet cats in Japan."J. Microbiol. Methods. 37. 284-285 (1999)

Maruyama, S.、Tanaka, T.、Sakai, T. 和 Katsube, Y.：“日本宠物猫中巴尔通体的流行情况。”J.

Maruyama, S.他7名: "Seroprevalence of Bartonella henselae and Toxoplasma gondii among healthy individuals in Thailand"J.Vet.Med.Sci:. 62(印刷中). (2000)

Maruyama, S. 和其他 7 人：“泰国健康个体中巴尔通体和弓形虫的血清流行率”J.Vet.Med.Sci：62（印刷中）。