Molecular epidemiology for protozoan diseases in domestic animals in Xin

我们用血清学和分子生物学技术调查了新疆维吾尔自治区家畜立克次体病和原虫病的流行情况。通过基于柠檬酸合酶基因的PCR和测序检测来自牛的蜱DNA样品的立克次体感染。从达氏血蜱中检测到4个阳性样品，与最近检测到的“立克次体”高度相似。用间接免疫荧光法检测了新疆地区家畜无形体和埃立克体的感染情况。嗜吞噬细胞无形体和查菲埃立克体抗体主要在阿勒泰、伊犁和喀什地区放牧的反刍动物中检出。记录抗体滴度高达1：320。这些结果表明，在这些地区饲养的反刍动物可能感染了一些种的无形体和埃立克体。马巴氏杆菌和B.调查了新疆西部地区驴的卡巴里氏菌感染情况。在喀什和伊犁地区共随机抽取93份驴血清进行B检测。equi和B.使用重组抗原通过ELISA检测caballi感染。在93份样本中，9份（9.6%）和36份（38.7%）样本为B阳性。马感染和B. caballi感染。此外，2份（2.2%）样本对两种B均呈阳性。equi和B. caballi感染这些结果表明，马巴贝斯虫病在新疆西部地区的驴群中可能广泛流行，本研究对几种家畜原虫病的特异性抗原进行了筛选和评价，如犬新孢子虫、B. caballi，B. equi，B. gibsoni和B.牛传染病利用这些重组抗原建立了ELISA、免疫层析等血清学诊断方法。

We investigated the prevalence of rickettsial and protozoan diseases by serological and molecular techniques in domestic animals in Xinjiang Uygur Autonomous Region, China. Tick DNA samples from cattle were examined for Rickettsia infection by citrate synthase gene-based PCR and sequencing. Four positive samples were detected from Haemaphysalis danieli and high levels of similarity were found with recently detected 'Candidatus Rickettsia principis.' Anaplasma and Ehrlichia infection in domestic animals in Xinjiang area were examined by using an indirect immunofluorescence assay. The antibodies for Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia. The prevalence of Babesia equi and B. caballi infections in donkeys in western Xinjiang was investigated. In total, 93 serum samples were randomly taken from donkeys in the Kashgar and Ili areas, and examined for B. equi and B. caballi infections by ELISA using recombinant antigens. Of the 93 samples, 9 (9.6%) and 36 (38.7%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 2 (2.2%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine babesiosis might be extensively prevalent in donkeys in western Xinjiang.We explored and evaluated several candidates of specific antigens for protozoan diseases in domestic animals such as Neospora caninum, B. caballi, B. equi, B. gibsoni and B. bovis infections. We also developed several serological diagnostic methods such as ELISA and Immunochromatographic test with those recombinant antigens.