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Molecular epidemiology for protozoan diseases in domestic animals in Xin

辛县家畜原虫病的分子流行病学研究

基本信息

批准号：
15405040
负责人：
MARUYAMA Soichi
金额：
$ 9.02万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

项目摘要

We investigated the prevalence of rickettsial and protozoan diseases by serological and molecular techniques in domestic animals in Xinjiang Uygur Autonomous Region, China. Tick DNA samples from cattle were examined for Rickettsia infection by citrate synthase gene-based PCR and sequencing. Four positive samples were detected from Haemaphysalis danieli and high levels of similarity were found with recently detected 'Candidatus Rickettsia principis.' Anaplasma and Ehrlichia infection in domestic animals in Xinjiang area were examined by using an indirect immunofluorescence assay. The antibodies for Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia. The prevalence of Babesia equi and B. caballi infections in donkeys in western Xinjiang was investigated. In total, 93 serum samples were randomly taken from donkeys in the Kashgar and Ili areas, and examined for B. equi and B. caballi infections by ELISA using recombinant antigens. Of the 93 samples, 9 (9.6%) and 36 (38.7%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 2 (2.2%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine babesiosis might be extensively prevalent in donkeys in western Xinjiang.We explored and evaluated several candidates of specific antigens for protozoan diseases in domestic animals such as Neospora caninum, B. caballi, B. equi, B. gibsoni and B. bovis infections. We also developed several serological diagnostic methods such as ELISA and Immunochromatographic test with those recombinant antigens.

采用血清学和分子技术调查新疆维吾尔自治区家畜立克次体和原虫病的流行情况。采用基于柠檬酸合酶基因的PCR和测序方法检测牛蜱的立克次体感染。从达涅利血蜱中检测到4个阳性样本，并发现与最近检测到的主要立克次体候选菌高度相似。采用间接免疫荧光法检测新疆地区家畜无原体和埃利希体感染情况。主要在阿尔泰、伊犁和喀什地区牧场饲养的反刍动物中检测到嗜吞噬细胞无原体和沙菲埃立体抗体。抗体滴度高达1:320。这些结果表明，这些地区饲养的反刍动物可能感染了某些种类的无原体和埃利希体。对新疆西部地区毛驴中马巴贝斯虫和卡巴贝斯虫的流行情况进行了调查。随机抽取喀什和伊犁地区驴血清93份，采用重组抗原ELISA检测马双歧杆菌和卡巴利双歧杆菌感染情况。93份样本中，分别有9份（9.6%）和36份（38.7%）呈马布氏杆菌感染阳性。此外，2份（2.2%）样本马布氏杆菌和caballi布氏杆菌均阳性。这些结果表明，马巴贝斯虫病可能在新疆西部驴群中广泛流行。我们探索和评估了几种候选的家畜原生动物疾病特异性抗原，如犬新孢子虫、卡巴利贝氏杆菌、马贝氏贝氏杆菌、gibsoni和牛贝氏贝氏杆菌感染。我们还开发了多种血清诊断方法，如ELISA和免疫层析检测。

项目成果

期刊论文数量（89）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

図説獣医寄生虫学

兽医寄生虫学插图

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
内田明彦;野上貞雄;黄鴻堅
通讯作者：
黄鴻堅

Molecular survey of Mycoplasma haemofelis and‘Candidatus Mycoplasma haemofelis'in cats in Yamaguchi and surrounding area.

山口及周边地区猫中血猫支原体和“候选血猫支原体”的分子调查。

DOI：
发表时间：
2004
期刊：
Journal of Veterinary Medical Science Vol.66
影响因子：
0
作者：
Inokuma H;Taroura S;Okuda M;Hisasue M;Itamoto K;Une S;Nakaichi M;Taura Y.
通讯作者：
Taura Y.

Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAGl.

使用重组截短的NcSAG1通过酶联免疫吸附测定对牛中的犬新孢子虫感染进行血清诊断。

DOI：
发表时间：
2003
期刊：
Vet. Parasitol. 118
影响因子：
0
作者：
Chahan;B.;Gaturaga;I.;Huang;X.;Min;L.;Fukumoto;S.;Hirata;H.;Nishikawa;Y.;Suzuki;H.;Sugimoto;C.;Igarashi;I.;Mikami;T.;Xuan;X.
通讯作者：
X.

Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats