Production of transgenic trees that are tolerate to environmental stresses for supply of forest tree resources in the future

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In this study, winter-induced galactinol synthase gene in xylem of Larix kaempferi driven by cauliflower mosaic virus 35S promoter were introduced into hybrid aspen (Populus tremula×P. alba) to examine the enhancement of freezing stress tolerance. In transgenic hybrid aspen, levels of raffinose in green leaf, bark and xylem tissues were increased as compared with those of wild type, but freezing tolerance was not increased in these tissues. However, freezing tolerance of leaves after cold acclimation was only increased slightly with increase in levels of raffinose content.