Study on overwintering mechanism of dormant buds of trees for sustainable production of woody biomass

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In this study, extraorgan-freezing and endodormancy release of larch dormant buds were studied for understanding overwintering mechanism of dormant buds of trees.Cryo-scanning electron microscopic study revealed that primordial cells were partially dehydrated at subzero temperatures and water remaining in primordial cells was kept in the deep supercooling state. In this tissue, accumulation of several carbohydrates such as pinitol and sucrose were detected as major components. However, it is difficult to explain that high freezing tolerance by deep supercooling capability of primordial tissue is determined by accumulation of major carbohydrates alone. Possible contribution of minor components such as supercooling-facilitating substances might be studied in larch dormant buds. In addition, proteomic analysis by using LC-MS/MS showed that only minor changes in soluble protein composition occurred in larch dormant buds within three weeks during endodormancy release.