Analysis of denitrification activity expression factor of denitrification hotspot at groundwater environment based on molecular ecology

为了寻找具有硝酸盐还原活性的反硝化热点，建立了一种通过硝酸盐氮氧稳定同位素比值分析评价反硝化活性的方法。从底土中提取DNA，并进行PCR-DGGE图谱分析反硝化热点特定的细菌生态系统。将反硝化热点附近的底土与富含硝酸盐的地下水进行培养，结果表明，两周内硝酸盐被还原。

We developed a method of evaluating denitrification activity by analysis of nitrogen and oxygen stable isotope ratio of nitrate to search denitrification hotspot which has nitrate reduction activity.We searched out denitrification hotspot from alluvium-diluvium interface and took subsoils. DNA was extracted from subsoil and PCR-DGGE profiles were made for analysis of denitrification hotspot-specific bacterial ecosystems. Subsoils near denitrification hotspot were incubated with nitrate-rich groundwater, results showed that nitrate was reduced within two weeks.