Construction of artificial poly-clonal gamma globulin to control HCV infection and development of assay system

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178 clones of artificial poly-clonal gamma globulin, which consist of VH, CH1 and hinge region, was constructed from cDNA originated from peripheral blood. The expressed recombinant proteins in E. coli, were extracted, purified and recovered in milligram order. SCGK/J mouse, which is an inflammatory disease model, although the strain is not a model of hepatic disease, were examined for therapeutic treatment with the recombinant poly-clonal gamma globulin. After the therapeutic treatment in some dose of recombinant protein, hemogram and some cytokines were assayed. The level of peripheral blood lymphocyte and some inflammatory cytokines were decreased in dose dependent manner, and some suppressive cytokines were increased. It was suggested that the recombinant poly-clonal gamma globulin, constructed in this study, would be possible to apply to the therapy for hepatic inflammatory disease.