Construction of artificial poly-clonal gamma globulin to control HCV infection and development of assay system

控制HCV感染的人工多克隆丙种球蛋白的构建及测定系统的开发

• 批准号: 20590117
• 负责人: KAMEOKA Yousuke
• 金额: $ 3万
• 依托单位: National Institute of Biomedical Innovation
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590117/
• 关键词: ゲノム創薬; 人体抗体; C型肝炎; HCV; 感染制御

178 clones of artificial poly-clonal gamma globulin, which consist of VH, CH1 and hinge region, was constructed from cDNA originated from peripheral blood. The expressed recombinant proteins in E. coli, were extracted, purified and recovered in milligram order. SCGK/J mouse, which is an inflammatory disease model, although the strain is not a model of hepatic disease, were examined for therapeutic treatment with the recombinant poly-clonal gamma globulin. After the therapeutic treatment in some dose of recombinant protein, hemogram and some cytokines were assayed. The level of peripheral blood lymphocyte and some inflammatory cytokines were decreased in dose dependent manner, and some suppressive cytokines were increased. It was suggested that the recombinant poly-clonal gamma globulin, constructed in this study, would be possible to apply to the therapy for hepatic inflammatory disease.

利用外周血cDNA构建了178个人工多克隆丙种球蛋白克隆，由VH、CH1和铰链区组成。将大肠杆菌中表达的重组蛋白以毫克级进行提取、纯化和回收。 SCGK/J小鼠是一种炎症性疾病模型，尽管该品系不是肝病模型，但仍对重组多克隆丙种球蛋白的治疗进行了检查。在一定剂量的重组蛋白治疗后，测定血象和一些细胞因子。外周血淋巴细胞和部分炎性细胞因子水平呈剂量依赖性下降，部分抑制性细胞因子水平升高。提示本研究构建的重组多克隆丙种球蛋白有望应用于肝脏炎症性疾病的治疗。

项目成果

モノバレントVH-CH1-h型人エガンマグロブリンの多様性と結合性の検討

单价 VH-CH1-h 型人丙种球蛋白的多样性和结合特性的检查

• 发表时间: 2009
• 作者: 亀岡洋祐;内田敬子;田中早苗;平田誠;田沼玲子;竹内昌男;竹内喜久子;長田直樹;高橋一朗1;三浦典子;大野尚仁;鈴木和男
• 通讯作者: 鈴木和男

改良型人工ガンマグロブリンの多様性と結合性の検討

改良人工丙种球蛋白多样性和连通性的研究

• 发表时间: 2009
• 作者: 亀岡洋祐;内田敬子;田中早苗;平田誠;田沼玲子;竹内昌男;竹内喜久子;長田直樹;高橋一朗;三浦典子;大野尚仁;鈴木和男
• 通讯作者: 鈴木和男

Development of an integrative database with 499 novel microsatellite markers for Macaca fascicularis

• DOI: 10.1186/1471-2156-10-24
• 发表时间: 2009-06-05
• 期刊: BMC GENETICS
• 影响因子: 2.9
• 作者: Higashino, Atsunori;Osada, Naoki;Terao, Keiji
• 通讯作者: Terao, Keiji

Ancient genome-wide admixture extends beyond the current hybrid zone between Macaca fascicularis and M-mulatta

• DOI: 10.1111/j.1365-294x.2010.04687.x
• 发表时间: 2010-07-01
• 期刊: MOLECULAR ECOLOGY
• 影响因子: 4.9
• 作者: Osada, Naoki;Uno, Yasuhiro;Terao, Keiji
• 通讯作者: Terao, Keiji

カニクイザル骨髄, 脾臓, 膵臓由来cDNAライブラリーの解析

食蟹猴骨髓、脾脏和胰腺的 cDNA 文库分析

• 发表时间: 2008
• 作者: 長田直樹;亀岡洋祐;平田誠;田沼玲子;鈴木穣;菅野純夫;高橋一朗
• 通讯作者: 高橋一朗