Research of drug-resistant mechanism in highly pathogenic avian influenza virus

高致病性禽流感病毒耐药机制研究

• 批准号: 20590118
• 负责人: TAGAWA Yuko
• 金额: $ 3.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590118/
• 关键词: 高病原性鳥インフルエンザウイルス; 薬剤耐性; 抗インフルエンザ薬; 迅速診断; 病原性; 鳥インフルエンザウイルス; オセルタミビル; 国際情報交換; ベトナム:インドネシア; 抗ウイルス剤

The persistence of H5N1 avian influenza viruses in many Asian countries and their ability to cause fatal infections in humans have raised serious concerns about a global flu pandemic. We obtained important results as below :(1) Oseltamivir-resistant H5N1 viruses have been isolated from oseltamivir-treated patients. The investigational drug T-705 has effectively protects mice from lethal infection with these viruses.(2) H5N1 viruses have been transmitted to pigs on multiple occasions in Indonesia.(3) A direct analysis of the viral RNAs of H5N1 viruses in patients revealed that efficient virus propagation in the human. Viruses grown in culture or eggs did not always reflect those in patients.(4) Many influenza rapid diagnostic tests are available for the detection of H5N1 influenza virus infections.

H5 N1禽流感病毒在许多亚洲国家的持续存在及其在人类中造成致命感染的能力引起了人们对全球流感大流行的严重关切。主要结果如下：（1）从奥司他韦治疗的患者中分离到对奥司他韦耐药的H5 N1病毒。研究药物T-705有效地保护小鼠免受这些病毒的致命感染。(2)在印度尼西亚，H5 N1病毒曾多次传播给猪。(3)对患者体内H5 N1病毒RNA的直接分析表明，病毒在人体内有效繁殖。培养物或鸡蛋中生长的病毒并不总是反映患者体内的病毒。(4)许多流感快速诊断测试可用于检测H5 N1流感病毒感染。

项目成果

The HA and NS genes of human H5N1 influenza A virus contribute to high virulence in ferrets.

• DOI: 10.1371/journal.ppat.1001106
• 发表时间: 2010-09-16
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Imai H;Shinya K;Takano R;Kiso M;Muramoto Y;Sakabe S;Murakami S;Ito M;Yamada S;Le MT;Nidom CA;Sakai-Tagawa Y;Takahashi K;Omori Y;Noda T;Shimojima M;Kakugawa S;Goto H;Iwatsuki-Horimoto K;Horimoto T;Kawaoka Y
• 通讯作者: Kawaoka Y

Sensitivity of Influenza Rapid Diagnostic Tests to H5N1 and 2009 Pandemic H1N1 Viruses

• DOI: 10.1128/jcm.00439-10
• 发表时间: 2010-08-01
• 期刊: JOURNAL OF CLINICAL MICROBIOLOGY
• 影响因子: 9.4
• 作者: Sakai-Tagawa, Yuko;Ozawa, Makoto;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Influenza A (H5N1) viruses from pigs, Indonesia.

• DOI: 10.3201/eid1610.100508
• 发表时间: 2010-10
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Nidom CA;Takano R;Yamada S;Sakai-Tagawa Y;Daulay S;Aswadi D;Suzuki T;Suzuki Y;Shinya K;Iwatsuki-Horimoto K;Muramoto Y;Kawaoka Y
• 通讯作者: Kawaoka Y

Pathogenicity of highly-pathogenic avian H5N1 influenza A viruses isolated from humans during 2003 to 2008 in Northern Vietnam

2003年至2008年越南北部从人类分离出的高致病性禽H5N1甲型流感病毒的致病性

• 发表时间: 2010
• 作者: 今井博貴;他19名;Le QM
• 通讯作者: Le QM

H5N1不活化インフルエンザワクチンのクレード間交差防御免疫能の解析

H5N1灭活流感疫苗进化枝间交叉保护免疫潜力分析

• 发表时间: 2008
• 作者: 村上晋;岩佐彩香;岩附研子;伊藤睦美;木曽真紀;喜田宏;高田礼人;CA Nidom;Le Quynh Mai;山田晋弥;今井博貴;坂井(田川)優子;河岡義裕;堀本泰介
• 通讯作者: 堀本泰介