Cell biological study of primary cilia in non-epithelial cells

用形态学和细胞生物学方法研究了输卵管粘膜和非上皮细胞的初代纤毛。初级纤毛轴突缺乏动力臂，不动。外周双链的移位被认为是由于缺乏连接蛋白连接。纤毛颈部的电子致密物质被认为是纤毛传输的屏障。培养成纤维细胞（如KD细胞）的原代纤毛延伸到细胞质囊泡中，在纤毛周鞘内观察到纤毛。纤毛膜的形态改变最初发生在纤毛脱落时。rabb家族是囊泡运输的关键调节因子，已知gtp结合的Rab8是纤毛膜生物发生所必需的。在转染Rab8a sirna的培养成纤维细胞中，约20%的细胞显示出巨大的原毛，这表明Rab8a与控制纤毛长度的分子的运输有关。纤毛微管、中心粒和收缩环通常被乙酰化。微管蛋白乙酰化酶被证实定位于中心体区域。

Primary cilia in the oviduct mucosa and non-epithelial cultured cells were investigated by morphological and cell biological methods. The axoneme of primary cilia were lacking dynein arms and immotile. Displacement of peripheral doublets was suggested to be attributable to the lack of nexin links. Electron dense materials at the ciliary neck were thought to work as a barrier of the ciliary transport. Primary cilia of cultured fibroblasts such as KD cells extended into cytoplasmic vesicles and observed as a cilium within a periciliary sheath. Morphological changes of the ciliary membrane initially occurred at deciliation. The Rab family is a key regulator of vesicle trafficking and it is known that GTP-bound Rab8 is required for the biogenesis of ciliary membrane. About 20% of cultured fibroblast transected with siRNAs of Rab8a displayed giant primary cilia, indicating that Rab8a is concerned with the transport of the molecules that control the ciliary length. Microtubules of cilia, centrioles, and contractile rings were usually acetylated. Tubulin acetylase were demonstrated to be localized to the centrosome region.