Study of the regulation mechanism of ciliogenesis

1.对人输卵管上皮细胞纤毛发生的超微结构进行了观察。在基底体和纤毛之间的过渡区形成了短管结构，称为经小管。小管直径34-36 nm，直径0.13(-)。[)长度0.06微米，在纤毛发生过程中短暂存在，被认为是在纤毛伸长过程中固定基底和/或在翼片形成中起重要作用。原始根束分为两个截然不同的部分，近端圆锥形部分与基底体相连，远端纤维部分。由于纤毛纤维部分在成熟的纤毛器官中不明显，在纤毛发生过程中该部分被认为是降解的或转变为圆锥形部分。2.通过向大鼠去纤毛细胞顶端皮质注射产生的单抗R4109进行鉴定，发现了一种与纹状根相关的新蛋白。R4109在免疫印迹中识别了一个58 kDa的蛋白，并在免疫荧光和免疫电子显微镜上修饰了条纹突起。R4109标记了输卵管和呼吸道纤毛细胞、室管膜细胞和视网膜感光细胞中的条纹细根。与孤立纤毛相关的条纹细根也被染色。R4109在PtK2、HF等培养细胞中可见条状根，未见孤立纤毛的形成。在纤毛形成细胞的免疫组织化学研究中，纤维颗粒被R4109修饰，强烈提示纤维颗粒与小根的形成有关。

1.I investigated the ultrastructure of, ciligenesis in the human oviduct epithelium. Short tubular structures, termed transitinal tubules, were formed in the transitional zone between the basal body and cilium. The tubules, 34-36 nm in diameter and 0.13 (]SY.+-。[)0.06mum in length, existed transiently during ciliogenesis and were considered, to fix the basal body during ciliary elongation and/or play an important role in alar sheet formation.Fibrous granules were deeply concerned with the formation of striated rootlets. Primitive rootlets were divided into two distinct parts, the proximal conical partconnected to the basal body and the distal fibrillar part. Since the fibrillar part was obscure in the mature ciliary apparatus, this part was considered as being eitherdegraded or changed into the conical part during ciliogenesis.2.A novel protein associated with striated rootlets were found by identifying a monoclonal antibody R4109 which was produced by injecting to apical cortices of deciliated cells to rats. R4109 recognized a 58 kDa protein on immunoblotting and decorated striated rootlets on immunofluorescence and immunoelectron microscopy. R4109 decorated striated rootlets in ciliated cells of the oviduct and respiratory tract, ependymal cells, and retinal photoreceptor cells.Striated rootlets associated with the solitary cilium were also stained. Striated rootlets were demonstrated by R4109 in culture cells such as PtK2 and HF without formation of the solitary cilium. Fibrous granules were decorated by R4109 in immunohistochemical investigation of ciliogenic cells, suggesting strongly that fibrous granules were concerned with rootlet formation.