The molecular mechanisms of the convergence in the early stage of the cell growth and adhesion signalings enhanced by glycosphingolipids

鞘糖脂增强细胞生长早期收敛和粘附信号的分子机制

• 批准号: 20590319
• 负责人: FURUKAWA Keiko
• 金额: $ 3万
• 依托单位: Chubu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590319/
• 关键词: 酸性スフィンゴ糖脂質; GD3; メラノーマ; インテグリン; 脂質ラフト; シグナル伝達; ヒトメラノーマ細胞; c-Met; HGF; Akt; 細胞膜脂質ラフト; I型コラーゲン; ILK; コラーゲンI; 細胞外マトリックス; p130Cas; paxillin

Glycosphingolipid, GD3 is widely expressed in human malignant melanoma cells and tumors. In this study, we analyzed the molecular mechanisms for the convergence in the initial stage of the cell growth and adhesion signalings enhanced by GD3. Our results suggested that the signaling molecules were phosphorylated through the growth factor receptors more strongly in GD3-expressing cells than in GD3 negative cells. This was also the case for signaling via cell adhesion probably due to the assembly and clustering of integrins in lipid rafts, leading to the malignant properties of melanomas under GD3 expression.

鞘糖脂GD3在人类恶性黑色素瘤细胞和肿瘤中广泛表达。在本研究中，我们分析了GD3增强细胞生长初期趋同和粘附信号的分子机制。我们的研究结果表明，在GD3表达细胞中，信号分子通过生长因子受体的磷酸化比在GD3阴性细胞中更强烈。这也是通过细胞粘附传递信号的情况，可能是由于脂筏中整合素的组装和聚集，导致GD3表达下黑色素瘤的恶性特性。

项目成果

Reduced motor and sensory functions and emotional response in GM3-only mice: Emergence from early stage of life and exacerbation with aging

• DOI: 10.1016/j.bbr.2008.10.024
• 发表时间: 2009-03-02
• 期刊: BEHAVIOURAL BRAIN RESEARCH
• 影响因子: 2.7
• 作者: Tajima, Orie;Egashira, Nobuaki;Furukawa, Koichi
• 通讯作者: Furukawa, Koichi

Immunologic glycosphingolipidomics and NKT cell development in mouse thymus.

• DOI: 10.1021/pr801040h
• 发表时间: 2009-06
• 期刊: Journal of proteome research
• 影响因子: 4.4
• 作者: Li Y;Thapa P;Hawke D;Kondo Y;Furukawa K;Furukawa K;Hsu FF;Adlercreutz D;Weadge J;Palcic MM;Wang PG;Levery SB;Zhou D
• 通讯作者: Zhou D

ヒト口腔扁平上皮癌におけるマーカーとしてのtype2HとLewisY抗原

2H 型和 LewisY 抗原作为人类口腔鳞状细胞癌的标志物

• 发表时间: 2010
• 作者: 堀田公司;浜村和紀;豊國伸哉;渋谷英伸;徳田典代;古川圭子;山本憲幸;服部 宇;上田実;古川鋼一
• 通讯作者: 古川鋼一

ヒトメラノーマ細胞における膜型シアリダーゼ(NEU3)の癌性形質への関与

膜型唾液酸酶（NEU3）参与人黑色素瘤细胞的癌性特征

• 发表时间: 2009
• 作者: 古川圭子;田島織絵;神戸真理子;宮田麻衣子;古川鋼一;宮城妙子
• 通讯作者: 宮城妙子

Molecular complex formation containing glycolipids for the regulation of signals.

含有糖脂的分子复合物形成用于调节信号。

• 发表时间: 2008
• 作者: Furukawa K.;Ohmi Y.;Ohkawa Y.;Kondo Y.;Zhang Q.;Furukwawa K.
• 通讯作者: Furukwawa K.