Molecular mechanisms for the regulation of T cell signals by sphingoglycolipids
鞘糖脂调节 T 细胞信号的分子机制
基本信息
- 批准号:10670118
- 负责人:
- 金额:$ 2.18万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
T cell development and function in complex ganglioside-lacking (GM2/GD2 synthase gene-disrupted) mice were analyzed. GM1, asialo-GM1 and gGD1b were representative gangliosides expressed on T cells of the wild type mice, and completely detected on those of mutant mice. The sizes and cell numbers of the mutant mice spleen and thymus were apparently reduced. When spleen cells were cultured with interleukin-2 (IL-2), those from the mutant showed reduced proliferation compared to those from the wild type. In contrast, proliferation by stimulation with concanavalin A or anti-CD3 cross-linking did not exhibit definite differences. Expression levels of IL-2 receptor α, β and γ were almost equivalent, and up-regulation of α chain after T cell activation were also similar between the mutant and wild type mice. Activation of JAK1, JAK3 and STAT5 after IL-2 treatment was reduced, and c-fos expression was delayed and reduced in the spleen cells of the mutant, suggesting that the IL-2 signal was attenuated in the mutant mice probably due to the modulation of the function of IL-2 receptors by the lack of complex gangliosides.
分析了复杂神经节苷脂缺乏(GM 2/GD 2合酶基因破坏)小鼠中的T细胞发育和功能。GM 1、去唾液酸GM 1和gGD 1b是在野生型小鼠的T细胞上表达的代表性神经节苷脂,并且在突变型小鼠的T细胞上完全检测到。突变小鼠脾脏和胸腺的体积和细胞数明显减少。当脾细胞与白细胞介素-2(IL-2)一起培养时,与野生型相比,突变体的脾细胞增殖减少。与此相反,增殖刺激伴刀豆球蛋白A或抗CD 3交联没有表现出明确的差异。突变型和野生型小鼠IL-2受体α、β和γ的表达水平几乎相等,T细胞活化后α链的上调也相似。IL-2处理后JAK 1、JAK 3和STAT 5的激活减少,突变小鼠脾细胞中c-fos表达延迟和减少,表明突变小鼠中IL-2信号减弱,可能是由于缺乏复合神经节苷脂调节IL-2受体的功能。
项目成果
期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Fukumoto, S et al.: "GD3 synthase gene expression in PC12 cells results in the continuous activation of TrkA and ERK1/2 and enhanced proliferation"J. Biol. Chem.. (in press).
Fukumoto, S 等人:“PC12 细胞中 GD3 合酶基因的表达导致 TrkA 和 ERK1/2 的持续激活并增强增殖”J.
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Okajima,T.: "Molecular cloning of brain-specific GD1αsynthase(ST6GalNAc V)containing CAG/glutamine repeats"J.Biol.Chem.. 274. 30557-30562 (1999)
Okajima, T.:“含有 CAG/谷氨酰胺重复序列的脑特异性 GD1α 合酶 (ST6GalNAc V) 的分子克隆”J.Biol.Chem.. 274. 30557-30562 (1999)
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Okajima, T et al.: "Molecular cloning and expression of mouse GD1α/GT1aα/GQ1bα synthase(ST6GalNAc VI) gene"J. Biol. Chem.. (in press).
Okajima, T 等人:“小鼠 GD1α/GT1aα/GQ1bα 合酶 (ST6GalNAc VI) 基因的分子克隆和表达”J. Biol。
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Alam Khorshed S.M.: "Expression and purification of a mutant human growth hormone that is resistant to proteolytic cleavage by thrombin, plasmin and human plasma in vitro." J.Biotechnol.65. 183-190 (1998)
Alam Khorshed S.M.:“一种突变型人类生长激素的表达和纯化,该激素在体外能够抵抗凝血酶、纤溶酶和人血浆的蛋白水解切割。”
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- 影响因子:0
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- 通讯作者:
Okajima,T.: "Molecular cloning and expression of mouse GD1α/GT1aα/GQ1bα synthase(ST6GalNAc VI)gene"J.Biol.Chem.. (in press).
Okajima, T.:“小鼠 GD1α/GT1aα/GQ1bα 合酶 (ST6GalNAc VI) 基因的分子克隆和表达”J.Biol.Chem..(印刷中)。
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2019 - 期刊:
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KAWATA Masayoshi;FUKUI Ayaka;MINEHARU Yohei;KIKUCHI Takayuki;YAMAO Yukihiro;YAMAMOTO HATTORI Etsuko;SHIRAKI Atsuko;MIZOTA Toshiyuki;FURUKAWA Keiko;MIYAMOTO Susumu;YONEZAWA Atsushi;ARAKAWA Yoshiki;武藤 淳 - 通讯作者:
武藤 淳
FURUKAWA Keiko的其他文献
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{{ truncateString('FURUKAWA Keiko', 18)}}的其他基金
Regulatory mechanisms for inflammatory responses and expression of cancer-associated glycosyltransferase genes by microenvironment factors, and their implications
微环境因素对炎症反应和癌症相关糖基转移酶基因表达的调节机制及其影响
- 批准号:
26460404 - 财政年份:2014
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Spatio-temporal analysis of cell membrane molecules involved in the enhancement of malignant properties by glycosphingolipids
鞘糖脂增强恶性特性涉及的细胞膜分子的时空分析
- 批准号:
23590371 - 财政年份:2011
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The molecular mechanisms of the convergence in the early stage of the cell growth and adhesion signalings enhanced by glycosphingolipids
鞘糖脂增强细胞生长早期收敛和粘附信号的分子机制
- 批准号:
20590319 - 财政年份:2008
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Roles of glycosphinglipids in converging process of the cell signals of proliferation and adhesion in cancer cells.
鞘糖脂在癌细胞增殖和粘附的细胞信号汇聚过程中的作用。
- 批准号:
18590291 - 财政年份:2006
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The mechanisms for the neuronal degeneration in the retina of the mutant mice lacking b-series acidic glycosphingolipids.
缺乏 b 系列酸性鞘糖脂的突变小鼠视网膜神经元变性的机制。
- 批准号:
16590243 - 财政年份:2004
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of the mechanism for neuronal degeneration and regeneration and responsible gene using the mutant mice disrupted of complex ganglioside
复合神经节苷脂破坏突变小鼠神经元变性再生机制及相关基因分析
- 批准号:
14570118 - 财政年份:2002
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanism for the maintenance and repair of nervous sptem with acidic
酸性维持和修复神经系统的分子机制
- 批准号:
12670111 - 财政年份:2000
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$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Gene therapy using promoters of glycosyltransferase genes which code tumor specific carbohydrate antigens.
使用编码肿瘤特异性碳水化合物抗原的糖基转移酶基因启动子的基因治疗。
- 批准号:
08670181 - 财政年份:1996
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analvsis of localization of a glycosyltransferase and transfort of glycolipids by gene manipulation
通过基因操作分析糖基转移酶的定位和糖脂的转移
- 批准号:
06670146 - 财政年份:1994
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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Ganglioside-CD44信号通路在PEMFs对小鼠缺血心肌血管生成影响中的作用及其机制研究
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