Clarification of liver condition progress mechanism that uses Hepatitis B virus reproduction model

使用乙型肝炎病毒繁殖模型阐明肝脏状况进展机制

• 批准号: 20590789
• 负责人: TANAKA Yasuhito
• 金额: $ 2.33万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590789/
• 关键词: HBV; 肝発癌; 共感染; キメラマウス; 免疫不全; トランスジェニックマウス; 遺伝子型; コアプロモーター; コア蛋白質; 遺伝子導入マウス; 肝線維化; モデルマウス

(1) Mechanism of hepatitis B virus (HBV) infection and replication : (1)HBV core protein as well as core promoter mutations are associated with viral replication. (2)The replication of genotype G was supported by core proteins of the other genotypes. (3)The hollow-fiber 3D culture system is superior to conventional monolayer culture allowing maintenance of HBV replication for a substantially longer duration. (2) Clarification of liver condition progress mechanism : Infection with HBV/C2 as well as PC mutant in immunosuppressive conditions can induce direct cytopathic effect in "humanized" part of the murine liver due to the activation of hepatic stellate cells mediated by oxidative stress.

(1)B型肝炎病毒（HBV）感染和复制的机制：（1）HBV核心蛋白及其核心启动子突变与病毒复制有关。（2）G基因型的复制受到其他基因型核心蛋白的支持。（3）中空纤维3D培养系统比常规单层培养优越上级，允许HBV复制维持实质上更长的持续时间。(2)阐明肝脏疾病进展机制：在免疫抑制条件下，HBV/C2以及PC突变体感染可在小鼠肝脏的“人源化”部分诱导直接细胞病变效应，这是由于氧化应激介导的肝星状细胞活化。

项目成果

B型肝炎に対するEntecavirの治療成績と各種血清マーカーの推移.

恩替卡韦治疗乙型肝炎的结果和各种血清标志物的趋势。

• 发表时间: 2009
• 作者: Kurbanov F;Tanaka Y;Simmonds P;Mizokami M.;(5人中2番目);Tanaka Yasuhito;Kurbanov Fuat;田中靖人;田中靖人;Shinkai Noboru;Sugiyama Masaya;Roman Sonia;Panduro Arturo;Shinkai Noboru;Sugiyama Masaya;Roman Sonia;Panduro Arturo;Fujiwara Kei;Matsuura Kentaroh;Fujiwara Kei;Matsuura Kentaroh;松浦健太郎
• 通讯作者: 松浦健太郎

Characteristics of hepatitis B virus genotype G coinfected with genotype H in chimeric mice carrying human hepatocytes

• DOI: 10.1016/j.virol.2008.04.001
• 发表时间: 2008-07-05
• 期刊: VIROLOGY
• 影响因子: 3.7
• 作者: Tanaka, Yasuhito;Sanchez, Laura V.;Mizokami, Masashi
• 通讯作者: Mizokami, Masashi

Direct Cytopathic Effects by Hepatitis B Virus Infection in uPA/SCID Mouse with Human Hepatocytes.

乙型肝炎病毒感染 uPA/SCID 小鼠与人肝细胞的直接细胞病变效应。

• 发表时间: 2009
• 作者: Kurbanov F;Tanaka Y;Simmonds P;Mizokami M.;(5人中2番目);Tanaka Yasuhito;Kurbanov Fuat;田中靖人;田中靖人;Shinkai Noboru;Sugiyama Masaya
• 通讯作者: Sugiyama Masaya

Direct Cytopathic Effects by Hepatitis B Virus Infection in uPA/SCID Mouse with Human Hepatocytes

乙型肝炎病毒感染 uPA/SCID 小鼠与人肝细胞的直接细胞病变效应

• 发表时间: 2009
• 作者: Kurbanov F;Tanaka Y;Simmonds P;Mizokami M.;(5人中2番目);Tanaka Yasuhito;Kurbanov Fuat;田中靖人;田中靖人;Shinkai Noboru;Sugiyama Masaya;Roman Sonia;Panduro Arturo;Shinkai Noboru;Sugiyama Masaya
• 通讯作者: Sugiyama Masaya

Clinical and virological characteristics of Hepatitis B Virus Genotype E: key mutation in seroconversion, and similarity of regularatory sequence to genotype D.

乙型肝炎病毒基因型 E 的临床和病毒学特征：血清转化的关键突变，以及与基因型 D 的规则序列的相似性。

• 发表时间: 2009
• 作者: Kurbanov F;Tanaka Y;Simmonds P;Mizokami M.;(5人中2番目);Tanaka Yasuhito;Kurbanov Fuat;田中靖人;田中靖人;Shinkai Noboru;Sugiyama Masaya;Roman Sonia;Panduro Arturo;Shinkai Noboru;Sugiyama Masaya;Roman Sonia;Panduro Arturo;Fujiwara Kei
• 通讯作者: Fujiwara Kei