Clarification of liver condition progress mechanism that uses Hepatitis B virus reproduction model

(1)乙型肝炎病毒（HBV）感染和复制的机制：(1)HBV核心蛋白及核心启动子突变与病毒复制有关。(2) G基因型的复制得到其他基因型核心蛋白的支持。(3)中空纤维3D培养系统优于传统的单层培养，可以维持HBV复制更长的时间。(2)澄清肝脏疾病进展机制：免疫抑制条件下感染HBV/C2和PC突变体可通过氧化应激介导的肝星状细胞活化，在小鼠肝脏“人源化”部分诱发直接的细胞病变作用。

(1) Mechanism of hepatitis B virus (HBV) infection and replication : (1)HBV core protein as well as core promoter mutations are associated with viral replication. (2)The replication of genotype G was supported by core proteins of the other genotypes. (3)The hollow-fiber 3D culture system is superior to conventional monolayer culture allowing maintenance of HBV replication for a substantially longer duration. (2) Clarification of liver condition progress mechanism : Infection with HBV/C2 as well as PC mutant in immunosuppressive conditions can induce direct cytopathic effect in "humanized" part of the murine liver due to the activation of hepatic stellate cells mediated by oxidative stress.